Normal mammalian secondary palate development undergoes a series of processes, including palatal shelf (PS) growth, elevation, adhesion and fusion, and palatal bone formation. It has been estimated that more than 90% of isolated cleft palate is caused by defects associated with the elevation process. However, because of the rapidly completed elevation process, the entire process of elevation will never be easy to clarify. In this article, we present a novel method for three-dimensional (3D) reconstruction of thick tissue blocks from two-dimensional (2D) histological sections. We established multiplanar sections of the palate and tongue in coronal and sagittal directions, and further performed 3D reconstruction to observe the morphological interaction and connection between the two components prior to and during elevation. The method completes an imaging system for simultaneous morphological analysis of thick tissue samples using both synthetic and real data. The new method will provide a comprehensive picture of reorientation morphology and gene expression pattern during the palatal elevation process.

正常的哺乳动物继发pa发育经历一系列过程，包括pa架（PS）生长，抬高，粘附和融合以及pa骨形成。据估计，超过90％的孤立c裂是由与抬高过程相关的缺陷引起的。但是，由于快速完成了高程过程，因此整个高程过程将永远不容易弄清楚。在本文中，我们提出了一种从二维（2D）组织切片对厚组织块进行三维（3D）重建的新方法。我们建立了冠状和矢状方向的上颚和舌头的多平面截面，并进一步进行了3D重建，以观察抬高之前和抬高期间两个部件之间的形态学相互作用和连接。该方法完成了一个成像系统，可同时使用合成数据和真实数据对厚组织样本进行形态学分析。新方法将提供pa上抬过程中重新定向形态和基因表达模式的全面图像。