Polymethyl methacrylate (PMMA) bone cement is a commonly used filling material in orthopedic surgery. Its good and stable performances make it the most widely used in all kinds of bone cement. With the advancement of the application and research of PMMA bone cement by surgeons, its disadvantages such as non-degradation and non-bioactivity are gradually exposed. In recent years, the biological functions of lactoferrin (LF) have been gradually explored, especially its role in promoting osteogenesis. In this study, LF was modified on the surface of solidified PMMA bone cement (LF/PMMA bone cement) by physical/chemical mixed modification and verified by cytological experiments in vitro. In vitro studies have implicated that PMMA bone cement modified with LF can improve the attachment, expansion, proliferation, extracellular matrix secretion and osteogenic differentiation of mouse preosteoblasts (MC3T3-E1) cells, indicating biocompatibility. This experiment provides a novel insight for improving the biological activity of PMMA bone cement and lays a foundation for broadening the clinical application of PMMA bone cement.

聚甲基丙烯酸甲酯（PMMA）骨水泥是骨科手术中常用的填充材料。其良好而稳定的性能使其在各种骨水泥中应用最为广泛。随着外科医生对PMMA骨水泥应用研究的不断深入，其不可降解、无生物活性等缺点逐渐暴露出来。近年来，人们逐渐探索了乳铁蛋白（LF）的生物学功能，特别是其促进成骨的作用。本研究通过物理/化学混合改性在固化的PMMA骨水泥（LF/PMMA骨水泥）表面改性LF，并通过体外细胞学实验验证。体外研究表明，经LF改性的PMMA骨水泥可改善附着、扩张、增殖、小鼠前成骨细胞 (MC3T3-E1) 细胞的细胞外基质分泌和成骨分化，表明生物相容性。该实验为提高PMMA骨水泥的生物活性提供了新的思路，为拓宽PMMA骨水泥的临床应用奠定了基础。