The morphology and proliferation of eukaryotic cells depend on their microenvironment. When electrospun mats are used as tissue engineering scaffolds, the local alignment of the fibers has a pronounced influence on cells. Here we analyzed the morphology of the patterned mats produced by electrospinning of PLA-gelatin blend onto a conductive grid. We investigated the cellular morphology and proliferation of two cell lines (keratinocytes HaCaT and fibroblasts NIH 3T3) on the patterned mats. The non-patterned mats of the same chemical composition were used as control ones. The HaCaT cells predominantly grew on convex areas of the patterned mats along with increasing their nucleus area and decreasing cell area. The 3T3 cells had a lower proliferative rate when grown on the patterned mats. The results can be valuable for further development of the procedures, which allow the patterned electrospun mats development as well as for the investigation of cell-substrate interactions.

真核细胞的形态和增殖取决于它们的微环境。当电纺垫用作组织工程支架时，纤维的局部排列对细胞有显着影响。在这里，我们分析了通过将 PLA-明胶混合物静电纺丝到导电网格上产生的图案垫的形态。我们研究了图案垫上两种细胞系（角质形成细胞 HaCaT 和成纤维细胞 NIH 3T3）的细胞形态和增殖。相同化学成分的无图案垫用作对照垫。HaCaT 细胞主要生长在图案垫的凸面区域，同时细胞核面积增加，细胞面积减少。3T3 细胞在图案垫上生长时具有较低的增殖率。