Abstract

To improve the naringinase production of Aspergillus tubingensis UA13, shorten the fermentation period, and verify its industrial application value, naringinase production conditions were optimized, and 5 L scale-up study in stirred tank bioreactor was carried out. Parameters, including carbon, nitrogen sources and inducer, optimal seed age, inoculum amount, temperature and pH, were adjusted and optimized in shaking flask. Keeping pH at the optimal value 6 in bioreactor, dissolved oxygen was monitored during the fermentation and the optimal stirring rate was investigated. In 5 L scale-up study, the highest naringinase activity was 72.62 U/mL, which was 1.75 times higher than that (41.52 U/mL) in shaking flask and the fermentation period was shortened by 24 h.

摘要

为提高图宾曲霉UA13柚皮苷酶产量，缩短发酵周期，验证其工业应用价值，优化柚皮苷酶生产条件，在搅拌罐生物反应器中进行5L放大研究。在摇瓶中对碳源、氮源和诱导剂、最佳种子年龄、接种量、温度和pH等参数进行了调整和优化。在生物反应器中保持最佳pH值为6，在发酵过程中监测溶解氧并研究最佳搅拌速率。在5 L放大研究中，柚皮苷酶的最高活性为72.62 U/mL，是摇瓶中（41.52 U/mL）的1.75倍，发酵周期缩短了24 h。