Abstract

Crystals of mutant carboxypeptidase T from Thermoactinomyces vulgaris (CPT11QG)with amino acid substitutions L211Q, T262S, L254S, and A251S were grown by the hanging-drop vapor-diffusion method. The crystals belong to sp. gr. P6(3)22. The X-ray-diffraction-data set,suitable for the crystal-structure determination at 2.6 Å resolution,was collected from the grown crystals at the ID23-1 beamline of the European Synchrotron Radiation Facility (ESRF, France).

中文翻译：

---

带有亲水化一级特异性口袋的突变型羧肽酶T的制备，结晶和X射线初步研究

摘要

通过悬滴气相扩散法生长了具有氨基酸取代基L211Q，T262S，L254S和A251S的寻常嗜热放线菌（CPT11QG）突变型羧肽酶T的晶体。晶体属于sp。gr。P 6（3）22。在欧洲同步辐射装置（法国ESRF）的ID23-1光束线处，从生长的晶体中收集了适用于2.6Å分辨率的晶体结构测定的X射线衍射数据集。