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Genome-wide Identification and Analysis of Late Embryogenesis Abundant (LEA) Genes in Musa acuminata
Tropical Plant Biology ( IF 2 ) Pub Date : 2021-05-25 , DOI: 10.1007/s12042-021-09289-0
Senuri Piyatissa , Dharshani Bandupriya

Key Message

Eighty four MaLEA genes were found coding for eighty five proteins, most of which are small and hydrophilic, and show ubiquitous distribution in the cell. MaLEA genes, having few introns, are distributed on all chromosomes, associated with abiotic stress-related cis-acting elements, and are differentially expressed under abiotic stress conditions.

Musa acuminata (Banana) is a major fruit crop grown worldwide. M. acuminata production is often threatened by abiotic environmental stresses. Late Embryogenesis Abundant (LEA) proteins play significant roles in plant growth, development, and responses to stresses. However, no studies have been carried out to elucidate the number, location, structure, molecular phylogenetics, and expression of LEA genes in M. acuminata. In this study, a genomic census of LEA genes in M. acuminata was carried out. A total of 84 LEA genes were identified and grouped in to eight sub-families. The LEA_2 subfamily was the largest and most diverse, including 57 members. Repeated motifs corresponding to LEA groups were present in the M. acuminata LEA proteins. The majority of MaLEA proteins was hydrophilic, and contained amino acids such as alanine, glycine and serine in abundance. Various functions were predicted for these proteins including response to stress, binding, structural components, and embryo development. The majority of MaLEA proteins are associated with the cytoplasm and chloroplast, while others are present in different subcellular compartments. MaLEAs have few introns (< 3) and are distributed unevenly across all chromosomes indicating that a significant portion of these genes originated by tandem or segmental duplications. The upstream sequences of these genes contain cis-acting elements associated with stress responses. The large number and diversity of LEA genes found in banana indicates that they are important in abiotic stress tolerance. This investigation provides valuable insights for future functional studies of MaLEA genes which may be useful for developing drought-tolerant banana varieties.



中文翻译:

全基因组识别和后期分析中的Mus acuminata晚期胚胎发生丰富(LEA)基因。

关键信息

发现八十四个MaLE A基因编码八十五种蛋白质,其中大多数是小而亲水的,并在细胞中普遍分布。具有较少内含子的MaLEA基因分布在所有染色体上,与非生物胁迫相关的顺式作用元件相关,并在非生物胁迫条件下差异表达。

Musa acuminata(香蕉)是世界范围内主要的水果作物。非生产性环境压力经常威胁着尖孢分枝杆菌的生产。晚期胚胎发生(LEA)蛋白质在植物生长,发育和对胁迫的反应中起重要作用。但是,没有研究已经进行了阐明的数量,位置,结构,分子系统,和表达的 LEA基因M.喜树。在这项研究中, 进行了M. acuminata LEA基因的基因组普查。总计84 LEA基因被鉴定并分为八个亚家族。LEA_2子家族最大,种类最多,包括57个成员。尖锐湿疣 LEA蛋白中存在对应于LEA基团的重复基序。MaLEA的大多数蛋白质都是亲水性的,并且富含氨基酸,如丙氨酸,甘氨酸和丝氨酸。预测了这些蛋白质的各种功能,包括对应激,结合,结构成分和胚胎发育的反应。MaLEA的大多数蛋白质与细胞质和叶绿体有关,而其他的蛋白质则存在于不同的亚细胞区室中。马来亚S的内含子很少(<3),并且在所有染色体上分布不均,表明这些基因的很大一部分是由串联或节段重复产生的。这些基因的上游序列包含与应激反应相关的顺式作用元件。香蕉中发现的LEA基因数量众多且具有多样性,表明它们在非生物胁迫耐受性中很重要。这项研究为MaLEA基因的未来功能研究提供了宝贵的见识,可能对开发耐旱香蕉品种有用。

更新日期:2021-05-25
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