Background

Mycobacterium tuberculosis (MTB) sRNAs are abundant. However, the level of MTB sRNA in peripheral blood remains elusive.

Methods

Twenty MTB sRNAs annotated in the reference genome of H37Rv were detected in the plasma of 170 active pulmonary tuberculosis patients and 124 healthy people by qRT-PCR detection system. The differential expression of sRNAs were analyzed in two groups. The value of sRNAs for diagnosis of active tuberculosis were evaluated by ROC curve analysis.

Results

Eight of the 20 sRNAs (MTS2823, MTS0997, MTS1338, ASdes, G2, C8, mcr15 and MTS1082) were found in at least 50% of the samples detected. The relative expression levels of MTS2823, MTS0997, MTS1338 and ASdes in plasma of tuberculosis patients were statistically higher than those in healthy controls. ROC curve analysis showed that the AUC of MTS0997, MTS1338, MTS2823 and ASdes were 0.8935 (95% CI 0.8109–0.9760), 0.8722 (95% CI 0.7862–0.9581), 0.8208 (95% CI 0.7246–0.9170) and 0.5792 (95% CI 0.4240–0.7344), respectively. The AUC value of combination of MTS0997, MTS1338 and MTS2823 was 0.914 (95% CI 0.8281–0.9926).

Conclusions

MTB sRNAs MTS2823, MTS0997 and MTS1338 have the potential to be plasma biomarkers for active pulmonary tuberculosis.

中文翻译：

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筛选血浆中 20 种结核分枝杆菌 sRNA 以检测活动性肺结核

背景

结核分枝杆菌(MTB) sRNA 含量丰富。然而，外周血中 MTB sRNA 的水平仍然难以捉摸。

方法

qRT-PCR检测系统在170名活动性肺结核患者和124名健康人的血浆中检测到20个注释在H37Rv参考基因组中的MTB sRNA。在两组中分析sRNA的差异表达。通过ROC曲线分析评价sRNAs对活动性肺结核的诊断价值。

结果

在至少 50% 的检测样本中发现了 20 种 sRNA 中的 8 种（MTS2823、MTS0997、MTS1338、ASdes、G2、C8、mcr15 和 MTS1082）。结核病患者血浆中MTS2823、MTS0997、MTS1338和ASdes的相对表达水平高于健康对照组。ROC曲线分析显示MTS0997、MTS1338、MTS2823和ASdes的AUC分别为0.8935（95% CI 0.8109-0.9760）、0.8722（95% CI 0.7862-0.9581）、0.8208（95% CI 0.7246-0.9170）和0.5792（5% CI 0.4240–0.7344），分别。MTS0997、MTS1338 和 MTS2823 组合的 AUC 值为 0.914（95% CI 0.8281–0.9926）。

结论

MTB sRNAs MTS2823、MTS0997 和 MTS1338 有可能成为活动性肺结核的血浆生物标志物。