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Organ-based chemo-profiling of Echinops echinatus Roxb. using high-performance thin-layer chromatography (HPTLC) technique
JPC - Journal of Planar Chromatography - Modern TLC ( IF 1.6 ) Pub Date : 2021-05-25 , DOI: 10.1007/s00764-021-00101-z
Nusrat Fayaz Bhatt , Raghbir Chand Gupta , Yogita Bansal

Plants contain different classes of secondary metabolites that have different levels of solubility in solvents, based on their polarity. The sequential extraction of Echinops echinatus was carried out using three solvent systems, namely water, hydromethanol, and chloroform, where the ratio of hydromethanol is (30:70V/V). The extracts were subjected to a standard phytochemical analysis against some specific marker compounds, which include the class of triterpenoids, phytosterols, and flavonoids. A varied range of phytochemicals were found in the extracts. Betulinic acid, oleanolic acid, and lupeol were found maximum in the hydromethanolic extract of the root samples (13.42 ± 0.01 mg/g, 10.50 ± 0.01 mg/g, and 9.69 ± 0.02 mg/g, respectively). Phytosterols like β-sitosterol, ergosterol, and campesterol were also found maximum in the hydromethanolic extract of the root samples (11.18 ± 0.01 mg/g, 13.12 ± 0.02 mg/g, and 8.83 ± 0.01 mg/g, respectively). Quercetin, caffeic acid, and chlorogenic acid were found maximum in the water extract of the leaf samples (14.00 ± 1.21 mg/g, 14.5 ± 0.24 mg/g, and 19.25 ± 0.01 mg/g). The present investigation on the medicinal plant E. echinatus has proved that the selection of solvent for extraction should be based on the target compounds. As in the present analysis, triterpenoids and phytosterols were found maximum in the hydromethanol and flavonoids were found maximum in the water extract.



中文翻译:

Echinops echinatus Roxb的基于器官的化学分析。使用高效薄层色谱(HPTLC)技术

植物包含不同种类的次生代谢物,根据其极性,它们在溶剂中的溶解度也不同。使用三种溶剂系统(水,氢甲醇和氯仿)对海胆紫锥菊进行顺序萃取,其中甲醇的比例为(30:70 V / V)。对提取物进行了针对某些特定标记化合物的标准植物化学分析,其中包括三萜类,植物甾醇和类黄酮。提取物中发现了各种各样的植物化学物质。在根样品的氢甲醇提取物中发现了桦木酸,齐墩果酸和羽扇豆酚含量最高(分别为13.42±0.01 mg / g,10.50±0.01 mg / g和9.69±0.02 mg / g)。在根样品的氢甲醇提取物中,植物甾醇(如β-谷固醇,麦角固醇和菜油甾醇)也最高(分别为11.18±0.01 mg / g,13.12±0.02 mg / g和8.83±0.01 mg / g)。在叶样品的水提取物中发现了槲皮素,咖啡酸和绿原酸含量最高(分别为14.00±1.21 mg / g,14.5±0.24 mg / g和19.25±0.01 mg / g)。药用植物的现状调查紫锥菊已证明提取溶剂的选择应基于目标化合物。如本分析中一样,三萜类化合物和植物甾醇在氢甲醇中含量最高,而黄酮含量在水提取物中含量最高。

更新日期:2021-05-25
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