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Self-regulation of cerebral metabolism and its neuroprotective effect after hypoxic-ischemic injury: evidence from 1H-MRS
Frontiers in Neuroanatomy ( IF 2.9 ) Pub Date : 2021-05-24 , DOI: 10.3389/fnana.2021.672412
Kexin Li 1 , Yang Zheng 1 , Xiaoming Wang 1
Affiliation  

1H-MRS technology can be used to noninvasively detect the content of cerebral metabolites, to assess the severity of hypoxic-ischemic (HI) injury, and to predict the recovery of compromised neurological function. However, changes to the cerebral self-regulation process after HI are still unclear. This study investigated the changes in cerebral metabolites and the potential relationship with the number of neurons and neural stem/progenitor cells (NSPC) using 1H-MRS, and finally clarifies the self-regulation of cerebral metabolism and neuroprotection after HI injury. Newborn Yorkshire pigs (28 males, 1.0–1.5 kg) aged 3–5 days were used for the HI model in this study. The pigs were randomly divided into the HI group (n = 24) and the control group (n = 4), then the experimental group was subdivided according to different recovery time after HI into the following groups: 0–2 h (n = 4), 2–6 h (n = 4), 6–12 h (n = 4), 12–24 h (n = 4), 24–48 h (n = 4), and 48–72 h (n = 4). Following the HI timepoints, 1H-MRS scans were performed and processed using LCModel software, and brain tissue was immunohistochemically stained for Nestin and NeuN. Immunofluorescence staining of creatine phosphokinase-BB (CK-BB), N-acetylaspartylglutamate synthetase (NAAGS), glutamate carboxypeptidase II (GCP-II), glutamate-cysteine ligase catalytic subunit (GCLC), glutathione synthase (GS), and excitatory amino acid carrier 1 (EAAC1) was then performed. The 1H-MRS results showed that cerebral N-acetylaspartylglutamate (NAAG), glutathione (GSH), and creatine (Cr) content reached their peaks at 12–24 h, which was consistent with the recovery time of hippocampal NSPCs and neurons, indicating a potential neuroprotective effect of NAAG, GSH, and Cr after HI injury.

中文翻译:

缺氧缺血性损伤后脑代谢的自我调节及其神经保护作用:1H-MRS的证据

1H-MRS技术可用于无创检测脑代谢产物的含量,评估缺氧缺血性(HI)损伤的严重程度,并预测受损的神经功能的恢复。但是,HI后大脑自我调节过程的变化仍不清楚。本研究利用1H-MRS研究了脑代谢产物的变化以及与神经元和神经干/祖细胞(NSPC)数量的潜在关系,并最终阐明了HI损伤后脑代谢的自我调节和神经保护作用。在本研究中,HI模型使用了3至5天的约克郡新生猪(28头雄性,1.0–1.5千克)。将猪随机分为HI组(n = 24)和对照组(n = 4),然后根据HI后不同的恢复时间将实验组分为以下几组:0–2 h(n = 4),2–6 h(n = 4),6–12 h(n = 4),12– 24小时(n = 4),24–48小时(n = 4)和48–72小时(n = 4)。在HI时间点之后,使用LCModel软件进行1H-MRS扫描和处理,并对脑组织进行Nestin和NeuN免疫组织化学染色。肌酸磷酸激酶-BB(CK-BB),N-乙酰天冬氨酰谷氨酸合成酶(NAAGS),谷氨酸羧肽酶II(GCP-II),谷氨酸半胱氨酸连接酶催化亚基(GCLC),谷胱甘肽合酶(GS)和兴奋性氨基酸的免疫荧光染色然后执行载体1(EAAC1)。1H-MRS结果显示,脑N-乙酰天冬氨酰谷氨酸(NAAG),谷胱甘肽(GSH)和肌酸(Cr)含量在12-24小时达到峰值,
更新日期:2021-05-25
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