Giardia lamblia, due to the habitat in which it develops, requires a continuous supply of intermediate compounds that allow it to survive in the host. The pentose phosphate pathway (PPP) provides essential molecules such as NADPH and ribulose-5-phosphate during the oxidative phase of the pathway. One of the key enzymes during this stage is 6-phosphogluconate dehydrogenase (6 PGDH) for generating NADPH. Given the relevance of the enzyme, in the present work, the 6pgdh gene from G. lamblia was amplified and cloned to produce the recombinant protein (Gl-6 PGDH) and characterize it functionally and structurally after the purification of Gl-6 PGDH by affinity chromatography. The results of the characterization showed that the protein has a molecular mass of 54 kDa, with an optimal pH of 7.0 and a temperature of 36–42 °C. The kinetic parameters of Gl-6 PGDH were K_m = 49.2 and 139.9 μM (for NADP⁺ and 6-PG, respectively), Vmax =26.27 μmol*min⁻¹*mg⁻¹, and Kcat = 24.0 s⁻¹. Finally, computational modeling studies were performed to obtain a structural visualization of the Gl-6 PGDH protein. The generation of the model and the characterization assays will allow us to expand our knowledge for future studies of the function of the protein in the metabolism of the parasite.

蓝氏贾第鞭毛虫，由于其生长的栖息地，需要持续供应中间化合物，使其能够在宿主中存活。磷酸戊糖途径 (PPP) 在该途径的氧化阶段提供必需分子，例如 NADPH 和 5-磷酸核酮糖。此阶段的关键酶之一是用于生成 NADPH 的 6-磷酸葡萄糖酸脱氢酶 (6 PGDH)。鉴于酶的相关性，在目前的工作中，来自G的6pgdh基因。兰布里亚扩增并克隆以产生重组蛋白 (Gl-6 PGDH)，并在通过亲和层析纯化 GI-6 PGDH 后对其进行功能和结构表征。表征结果表明，该蛋白质的分子量为 54 kDa，最适 pH 值为 7.0，温度为 36–42 °C。GI-6 PGDH 的动力学参数为 K _m = 49.2 和 139.9 μM（分别用于 NADP ⁺和 6-PG），Vmax =26.27 μmol*min ^-1 *mg ^-1和 Kcat = 24.0 s ^-1. 最后，进行计算建模研究以获得 GI-6 PGDH 蛋白的结构可视化。模型的生成和表征分析将使我们能够扩展我们的知识，以便将来研究蛋白质在寄生虫代谢中的功能。