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The combined use of Bacillus subtilis-based probiotic and anticoccidial herb had a better anti-Eimeria tenella efficiency
Journal of Applied Poultry Research ( IF 1.9 ) Pub Date : 2021-05-23 , DOI: 10.1016/j.japr.2021.100181
Y. Yang , F.U. Memon , K. Hao , M. Jiang , L. Guo , T. Liu , F. Lv , W. Zhang , Y. Zhang , H. Si

The objective of this work is to investigate the feed supplementation with Bacillus subtilis-based probiotic and methanol extraction of anticoccidial herbs (leaves of Artemisia annua Linn., Dichroa febrifuga Lour., and peel of Punica granatum L.) individually or in combination as alternatives during chicken E. tenella infection. A total of 264 chickens were divided into 11 groups (n = 8 × 3). They were control group (G1), E. tenella infected group (G2), E. tenella infected with diclazuril treated group (G3). G4 to G6 were E. tenella infected and treated with 300 mg/kg body weight of methanol extract of herb in feed (3 different kinds of herbs: leaves of Artemisia annua Linn., Dichroa febrifuga Lour., and peel of Punica granatum L.), respectively. G7 to G9 were infected and probiotic-herb combined groups: 5 × 108 cfu/kg B. subtilis was added to feed on the basis of G4 to G6, respectively. The remaining groups are both feed with B. subtilis at 5 × 108 cfu/kg in feed but infected (G10) and uninfected (G11). The anticoccidial efficacy was assessed by: bloody feces scores, lesion scores, relative weight gain rate, oocysts excretion, and mRNA expression levels (occludin, ZO-1, IL-17A and IL-2) in ceca. All the interventions reduced all the indexes, as compared to that of G2. The use of B. subtilis combined with herb significantly reduced the oocysts excretion and IL-17A mRNA level as compared to herb only groups. In summary, we conclude that the combination of B. subtilis and anticoccidial herb have potential development value.



中文翻译:

枯草芽孢杆菌为基础的益生菌与抗球虫药联合使用具有更好的抗艾美球虫效果

这项工作的目的是研究以枯草芽孢杆菌为基础的益生菌和甲醇提取物的抗球虫草本植物(青蒿、Dichroa febrifuga Lour.石榴果皮)单独或组合作为替代品的饲料补充剂在鸡E.tenella感染期间。总共 264 只鸡被分为 11 组(n = 8 × 3)。他们对照组(G1),柔嫩艾美耳球虫感染组(G2),柔嫩艾美耳球虫感染克珠利组(G3)。G4 到 G6 是感染E. tenella并在饲料中用 300 mg/kg 体重的草本甲醇提取物处理(3 种不同的草本:Artemisia annua Linn.、Dichroa febrifuga Lour.Punica granatum L. 的果皮)。G7-G9感染和益生菌-草本联合组:在G4-G6的基础上分别加入5×10 8 cfu/kg枯草芽孢杆菌饲料。其余组均以5 × 10 8 cfu/kg 的枯草芽孢杆菌喂养,但已感染(G10)和未感染(G11)。抗球虫药效通过以下评估:血便评分、病变评分、相对增重率、卵囊排泄和盲肠中的mRNA 表达水平(occludin、ZO-1、IL-17AIL-2)。与G2相比,所有干预措施都降低了所有指标。指某东西的用途与仅草药组相比,枯草芽孢杆菌与草药组合显着降低了卵囊排泄和IL-17A mRNA 水平。总之,我们得出结论,枯草芽孢杆菌与抗球虫药的组合具有潜在的开发价值。

更新日期:2021-06-29
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