Abstract

To the best of our knowledge, this is the first study on the effect of solvent (water, methanol, and ethyl acetate) upon phenolics extracted from Onosma mitis. The antioxidant capacities together with the enzyme inhibitory properties toward tyrosinase and α-amylase were also assessed. The yields of the extract were 2.62, 10.93, and 21.68% for ethyl acetate, methanol, and water, respectively. The concentrations of phenolic compounds were from 11.89 (ethyl acetate) to 2.66 mg gallic acid equivalent/g extract (methanol). Twenty-five molecules were identified and quantified by liquid chromatography – electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). Significant differences were present between the extracts; methanol presented the highest number of compounds (25) while ethyl acetate provided the lowest (18). Chlorogenic acid, luteolin 7-glucoside, rosmarinic acid, and apigenin 7-glucoside were the major compounds regardless of solvent. Antioxidant and inhibitory enzyme assays showed that O. mitis exhibited interesting biological properties. The results illustrate the use of O. mitis as a promising source of biomolecules for industrial applications.

摘要

据我们所知，这是第一次研究溶剂（水、甲醇和乙酸乙酯）对从Onosma mitis中提取的酚类物质的影响. 还评估了抗氧化能力以及对酪氨酸酶和α-淀粉酶的酶抑制特性。对于乙酸乙酯、甲醇和水，提取物的产率分别为 2.62、10.93 和 21.68%。酚类化合物的浓度为 11.89（乙酸乙酯）至 2.66 mg 没食子酸当量/g 提取物（甲醇）。通过液相色谱-电喷雾电离串联质谱 (LC-ESI-MS/MS) 鉴定和量化了 25 个分子。提取物之间存在显着差异；甲醇提供的化合物数量最多 (25)，而乙酸乙酯提供的化合物数量最少 (18)。无论溶剂如何，绿原酸、木犀草素 7-葡萄糖苷、迷迭香酸和芹菜素 7-葡萄糖苷都是主要化合物。抗氧化和抑制酶测定表明，O.mitis表现出有趣的生物学特性。结果说明了将 O. mitis用作工业应用的有希望的生物分子来源。