The bioengineering electroactive construct of a nerve-guided conduit for repairing and restoring injured nerves is an exciting biomedical endeavor that has implications for the treatment of peripheral nerve injury. In this study, we report the development the polycaprolactone (PCL) nanofibrous substrate consisting of turmeric (TUR) and polyaniline nanoparticles (PANINPs) exhibits topological and biological features that mimics the natural extracellular matrix (ECM) for nerve cells. We evaluated the morphology of 2-dimensional (2D) fibrous substrates, and their ability of stem cell adhesion, growth and proliferation rate were influenced by use of various concentrations of turmeric in PCL–TUR substrates. The results showed that 0.62 wt% of TUR and 0.28 wt% of PANINPs in PCL nanofibers substrate exhibited the optimal cellular microenvironment to accelerate PC12 cellular activities. The in vitro experiments revealed that PCL–TUR@PANI substrates significantly stimulated the proliferation, differentiation, and spontaneous outgrowth and extension of neurites from the cells. The substrate has the capacity to respond directly to neuronal markers with significant upregulation of βIII-Tubulin and TREK-1 through myelination, and also trigger neurotrophic protein expression, which was confirmed via immunocytochemistry and quantitative real-time polymerase chain reaction (qRT-PCR) analysis. This study provides a new technique to design substrate of nerve tissue-specific microenvironment for peripheral nerve cell regeneration and could offer promising biomaterials for in vivo peripheral nerve repair.

用于修复和恢复受伤神经的神经引导导管的生物工程电活性结构是一项令人兴奋的生物医学努力，对周围神经损伤的治疗具有重要意义。在这项研究中，我们报告了由姜黄 (TUR) 和聚苯胺纳米颗粒 (PANINPs) 组成的聚己内酯 (PCL) 纳米纤维基材的发展，该基材具有模拟神经细胞天然细胞外基质 (ECM) 的拓扑和生物学特征。我们评估了二维 (2D) 纤维基质的形态，它们的干细胞粘附能力、生长和增殖率受到在 PCL-TUR 基质中使用不同浓度姜黄的影响。结果表明，0.62wt%的TUR和0. PCL 纳米纤维基材中 28 wt% 的 PANINPs 表现出最佳的细胞微环境以加速 PC12 细胞活动。体外实验表明，PCL-TUR@PANI 底物显着刺激了细胞神经突的增殖、分化以及自发生长和延伸。底物能够直接响应神经元标记物，通过髓鞘形成显着上调 βIII-微管蛋白和 TREK-1，并触发神经营养蛋白表达，这已通过免疫细胞化学和定量实时聚合酶链反应 (qRT-PCR) 得到证实分析。该研究为设计用于周围神经细胞再生的神经组织特异性微环境基质提供了一种新技术，可为体内周围神经修复提供有前景的生物材料。