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Advances in the diagnosis of autoimmune diseases based on citrullinated peptides/proteins
Expert Review of Molecular Diagnostics ( IF 5.1 ) Pub Date : 2021-06-07 , DOI: 10.1080/14737159.2021.1933946
Mohammed F Alghamdi 1, 2 , Elrashdy M Redwan 1, 3
Affiliation  

ABSTRACT

Introduction: Autoimmune diseases are still one of the hard obstacles associated with humanity. There are many exogenous and endogenous etiological factors behind autoimmune diseases, which may be combined or dispersed to stimulate the autoimmune responses. Protein citrullination represents one of these factors. Harnessing specific citrullinated proteins/peptides could early predict and/or diagnose some of the autoimmune diseases. Many generations of diagnostic tools based on citrullinated peptides with comparable specificity/sensitivity are available worldwide.

Areas covered: In this review, we discuss the deimination reaction behind the citrullination of most known autoantigens targeted, different generations of diagnostic tools based on citrullinated probes with specificity/sensitivity of each as well as newly developed assays. Furthermore, the most advanced molecular analytical tools to detect the citrullinated residues in the biological fluid and their performance are also evaluated, providing new avenues to early detect autoimmune diseases with high accuracy.

Expert opinion: With the current specificity/sensitivity tools available for autoimmune disease detection, emphasis must be placed on developing more advance and effective, early, rapid, and simple diagnostic devices for autoimmune disease monitoring (similar to a portable device for sugar test at home). The molecular analytical devices with dual and/or multiplexe functions should be more simplified and invested in clinical laboratories.



中文翻译:

基于瓜氨酸肽/蛋白的自身免疫性疾病诊断进展

摘要

简介:自身免疫性疾病仍然是与人类相关的硬障碍之一。自身免疫性疾病背后有许多外源性和内源性的病因,它们可能结合或分散以刺激自身免疫反应。蛋白质瓜氨酸化代表这些因素之一。利用特定的瓜氨酸蛋白/肽可以早期预测和/或诊断一些自身免疫性疾病。多代基于瓜氨酸肽的诊断工具具有相当的特异性/敏感性,可在全球范围内使用。

涵盖的领域:在这篇综述中,我们讨论了大多数已知的靶向自身抗原瓜氨酸化背后的脱亚胺反应,基于瓜氨酸化探针的不同代诊断工具以及新开发的检测方法具有特异性/敏感性。此外,还评估了检测生物体液中瓜氨酸残留物的最先进分子分析工具及其性能,为早期高精度检测自身免疫性疾病提供了新途径。

专家意见:以目前可用于自身免疫性疾病检测的特异性/敏感性工具,必须将重点放在开发更先进、更有效、早期、快速和简单的自身免疫性疾病监测诊断设备(类似于家庭中用于糖检测的便携式设备) )。具有双重和/或多重功能的分子分析设备应更加简化并投资于临床实验室。

更新日期:2021-07-13
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