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RM12 similar to substance P from tachykinin of freshwater murrel Channa striatus influence intracellular ROS in vitro fish erythrocytes and developmental toxicity and antioxidant enzymes in vivo zebrafish embryo
Fish Physiology and Biochemistry ( IF 2.9 ) Pub Date : 2021-05-21 , DOI: 10.1007/s10695-021-00950-9
Stefi V Raju 1, 2 , Arnab Mukherjee 2 , Purabi Sarkar 1, 2 , Praveen Kumar Issac 1 , Christy Lite 3 , Bilal Ahmad Paray 4 , Mohammad K Al-Sadoon 4 , Abdul Rahman Al-Mfarij 4 , Jesu Arockiaraj 1, 2
Affiliation  

In this study, substance P, an antioxidant peptide of tachykinin, was identified using bioinformatics tools from the earlier established muscle transcriptome of a freshwater murrel Channa striatus and the peptide was named RM12. The antioxidant properties of RM12 were screened using various colorimetric assays. The toxicity of RM12 was experimented using fish erythrocytes, and it is observed that the maximum concentration (320 μM) of RM12 was found to have 15 or 20% of hemolytic activity; however, it was not significant with other tested concentrations (10, 20, 40, 80, and 160 μM). Further, the in vivo antioxidant properties of RM12 were experimented on zebrafish embryo, the intracellular ROS level was estimated by 5 mM H2O2 stress in the zebrafish embryo, and inhibition of apoptosis was evaluated. The antioxidant enzymes were extracted from the H2O2-stressed zebrafish embryo, and the intracellular ROS was eliminated due to RM12. Collectively, the experiment showed that the substance P from the freshwater murrel C. striatus possessed potent antioxidant properties; thus, it can further be focused to develop it as antioxidant molecule in aquaculture organisms.



中文翻译:

RM12 类似于来自淡水鲳鱼速激肽的 P 物质 Channa striatus 影响体外鱼红细胞内的 ROS 和体内斑马鱼胚胎的发育毒性和抗氧化酶

在这项研究中,物质 P 是一种速激肽的抗氧化肽,使用生物信息学工具从较早建立的淡水鳐鱼Channa striatus的肌肉转录组中鉴定出来,该肽被命名为 RM12。使用各种比色测定法筛选 RM12 的抗氧化特性。RM12的毒性用鱼红细胞进行实验,发现RM12的最大浓度(320 μM)被发现有15%或20%的溶血活性;然而,它与其他测试浓度(10、20、40、80 和 160 μM)无关。此外,RM12 的体内抗氧化特性在斑马鱼胚胎上进行了实验,细胞内 ROS 水平通过 5 mM H 2 O 2估计对斑马鱼胚胎的应激和细胞凋亡的抑制进行了评估。从H 2 O 2胁迫的斑马鱼胚胎中提取抗氧化酶,并通过RM12消除细胞内ROS。总的来说,实验表明淡水鲳鱼中的 P 物质具有强大的抗氧化特性;因此,可以进一步专注于将其开发为水产养殖生物中的抗氧化分子。

更新日期:2021-05-22
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