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Effects of Tiarella polyphylla D. Don Callus Extract on Photoaging in Human Foreskin Fibroblasts Hs68 Cells
Natural Product Communications ( IF 1.8 ) Pub Date : 2021-05-20 , DOI: 10.1177/1934578x211016970
Ha-Rim Kim 1 , Sol Kim 1 , Eun Yee Jie 2 , Sang Jun Kim 1 , Woo Seok Ahn 2 , Seung-Il Jeong 1 , Kang-Yeol Yu 1 , Suk Won Kim 2 , Seon-Young Kim 1
Affiliation  

Ultraviolet (UV)-irradiation causes an overproduction of matrix metalloproteinases (MMPs) and collagen (COL) degradation causing skin sunburn, inflammation, or photoaging in human. In this study, we prepared callus from Tiarella polyphylla D. Don (T. polyphylla) stem and its phytochemical profiles were analyzed using HPLC-MWD. The effects of T. polyphylla callus extract evaluated against on UVB-induced damage in human foreskin fibroblast (Hs68). Hs68 was exposed to UVB in the presence or absence of T. polyphylla callus extract at concentrations of 100 and 250 µg/mL. Cell damage caused by UVB was inhibited by T. polyphylla callus extract, which was tested by cell viability and caspase 3 activity in Hs68 cells. Further experiment revealed that T. polyphylla extract suppressed the level of MMP-1, but increased the level of type I procollagen. In addition, T. polyhylla callus extract inhibited UVB-mediated COL (-1 and -3) protein degradation and MMP (-1, 2, and -3) overexpression in Hs68. These results suggest that T. polyphylla callus extract has considerable potential as a cosmetic ingredient with anti-aging effects.



中文翻译:

的影响黄水枝属重楼在人包皮成纤维细胞的Hs68细胞D.唐愈伤组织提取物对皮肤光老化

紫外线(UV)照射会导致基质金属蛋白酶(MMP)和胶原蛋白(COL)过度生产,从而导致人体皮肤晒伤,发炎或光老化。在这项研究中,我们从多叶Ti草T. polyphylla D. Don)(T. polyphylla)茎中制备了愈伤组织,并使用HPLC-MWD分析了其植物化学特征。的效果T.一枝愈伤组织提取物在人包皮成纤维(的Hs68)对评价了UVB诱导的损伤。在存在和不存在浓度为100和250 µg / mL的多叶紫苏愈伤组织提取物的情况下,将Hs68暴露于UVB中。毛白杨(T. polyphylla)抑制UVB引起的细胞损伤。愈伤组织提取物,已通过Hs68细胞中的细胞活力和caspase 3活性进行了测试。进一步的实验表明,多叶紫茎泽兰提取物抑制了MMP-1的水平,但增加了I型胶原蛋白的水平。此外,T。polyhylla愈伤组织提取物抑制Hs68中UVB介导的COL(-1和-3)蛋白降解和MMP(-1、2和-3)过表达。这些结果表明,多叶红豆杉愈伤组织提取物作为具有抗衰老作用的化妆品成分具有相当大的潜力。

更新日期:2021-05-22
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