ABSTRACT

Arbuscular mycorrhizal fungi (AMF) can regulate the absorption of micro- and macro-nutrients and promote the growth and development of plants. The purpose of this study was to investigate the effect of AMF colonization on the Si absorption of tomato. Through potted planting experiment in the greenhouse, the symbiotic system of tomato seedlings and AMF, including Glomus versiforme (Gv), Funneliformis mosseae (Fm) and Acaulospora delicata (Ad), was established. The colonization rate, growth indicators and the Si content and concentration were analyzed. Different extractants were used to extract and determine the different Si-fractions in the rhizosphere soil. This study showed that the inoculation of Gv, Fm and Ad could promote the growth and increase the Si content in roots and shoots of tomato seedlings, and differences were found between AMF strains. Concentration of different binding states of Si in tomato rhizosphere soil was in descending order as citric acid-Si > NaOAc-HAc-Si > Oxalic acid-ammonium oxalate-Si > Na₂CO₃-Si > Na₂HCO₃-Si. When the tomato rhizosphere soils inoculated with various AMFs were extracted with the same extractant, the Si-fraction concentration changed with different AMF inoculations. AMF inoculation could regulate the conversion of different Si-fractions in the rhizosphere soil, which may be an important mechanism of AMF regulating the Si absorption of the tomato.

摘要

丛枝菌根真菌（AMF）可以调节微量和大量营养素的吸收，促进植物的生长发育。本研究的目的是研究 AMF 定植对番茄 Si 吸收的影响。通过温室盆栽试验，番茄幼苗与AMF共生系统，包括Glomus versiforme (Gv)、Funneliformis mosseae (Fm)和Acaulospora delicata（广告），成立。分析定植率、生长指标和Si含量和浓度。不同的萃取剂被用来萃取和测定根际土壤中不同的硅组分。本研究表明，接种Gv、Fm和Ad可以促进番茄幼苗根和芽的生长，增加Si含量，AMF菌株之间存在差异。Si在番茄根际土壤中不同结合态的浓度从高到低依次为柠檬酸-Si>NaOAc-HAc-Si>草酸-草酸铵-Si>Na ₂ CO ₃ -Si> Na ₂ HCO ₃-Si。接种不同AMFs的番茄根际土壤用相同提取剂提取时，不同AMF接种量的Si组分浓度发生变化。AMF接种可以调节根际土壤中不同Si组分的转化，这可能是AMF调节番茄对Si吸收的重要机制。