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Copper impair autophagy on zebrafish (Danio rerio) gill epithelium
Environmental Toxicology and Pharmacology ( IF 4.3 ) Pub Date : 2021-05-21 , DOI: 10.1016/j.etap.2021.103674
A Luzio 1 , S Parra 1 , B Costa 2 , D Santos 1 , A R Álvaro 3 , S M Monteiro 1
Affiliation  

Copper (Cu) is an essential element for organism's metabolism, being controversially listed as a priority pollutant. Importantly, the toxicity of Cu has been linked to several cell death pathways. Thus, this study aimed to assess if macroautophagic pathways are triggered by Cu in zebrafish gill, the main target of waterborne pollutants. The electron microscopy findings indicated that Cu induced profound impacts on zebrafish gill structure and functions, being this tissue a biomarker sensitive enough to indicate early toxic effects. The findings also support a clear impairment of autophagy, througth the absence of phagossomes and the significant down-regulation mRNA transcript levels of microtubule-associated protein light chain 3 (LC3). The reduction of LC3 levels was often associated to an increase of apoptotic activation, indicating that the inhibition of macroautophagy triggers apoptosis in zebrafish gills. This study highlighted that the autophagic down-regulation might be affected through the activation of other cell death signaling pathway.



中文翻译:

铜损害斑马鱼 (Danio rerio) 鳃上皮的自噬

铜 (Cu) 是生物体新陈代谢的必需元素,被列为优先污染物而备受争议。重要的是,Cu 的毒性与多种细胞死亡途径有关。因此,本研究旨在评估斑马鱼鳃中的铜是否会触发巨自噬途径,斑马鱼鳃是水传播污染物的主要目标。电子显微镜结果表明,Cu 对斑马鱼鳃结构和功能产生了深远的影响,因为这种组织是一种足够敏感的生物标志物,可以表明早期的毒性作用。这些发现还支持自噬明显受损,原因是吞噬体的缺失和微管相关蛋白轻链 3 ( LC3 ) 的mRNA 转录水平显着下调。LC3的减少水平通常与细胞凋亡激活的增加有关,表明抑制巨自噬会触发斑马鱼鳃的细胞凋亡。该研究强调自噬下调可能通过激活其他细胞死亡信号通路受到影响。

更新日期:2021-05-25
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