Abstract

In recent years, interactions of radiofrequency (RF) electromagnetic fields with biological tissue have become one of the most promising strategies for noninvasive cancer hyperthermia treatment. Despite the growing interest, there has been a scarcity of studies involving the detailed construction of a complete RF-hyperthermia (RF-HT) system. Here a low-cost and high-power RF-HT system is reported with a specific frequency which can be used in biological samples. A laboratory-constructed RF-HT system composed of a radiofrequency oscillator, radiofrequency driver, radiofrequency amplifier, radiofrequency matching network, and an induction coil applicator were constructed and characterized by a stepwise approach. Although the RF driver and amplifier were purchased as professionally designed kits, significant modifications were required for these components. The results showed that the RF-HT system was successfully constructed and tested at 13.56 MHz with a power as high as 400 W. As a preliminary biological experiment, cell culture medium exhibited an approximate 3 °C increase in temperature induced by the RF-HT device at 250 W for 10 min. Moreover, the developed system is suitable drug targeting, drug release, and cellular uptake and designed to be cost-effective for in vitro/in vivo studies involving cancer therapy.

摘要

近年来，射频 (RF) 电磁场与生物组织的相互作用已成为无创癌症热疗治疗最有前途的策略之一。尽管兴趣日益浓厚，但涉及完整射频热疗 (RF-HT) 系统的详细构建的研究很少。这里报道了一种具有特定频率的低成本和高功率 RF-HT 系统，可用于生物样品。实验室构建的RF-HT系统由射频振荡器、射频驱动器、射频放大器、射频匹配网络和感应线圈施加器组成，并通过逐步方法进行表征。虽然 RF 驱动器和放大器是作为专业设计的套件购买的，这些组件需要进行重大修改。结果表明，RF-HT 系统成功构建并在 13.56 MHz 下测试，功率高达 400 W。作为初步生物学实验，RF-HT 诱导细胞培养基温度升高约 3 °C。设备在 250 W 下持续 10 分钟。此外，所开发的系统适用于药物靶向、药物释放和细胞摄取，并且具有成本效益。涉及癌症治疗的体外/体内研究。