ABSTRACT

Introduction: Respiratory syncytial virus (RSV) is a major human pathogen associated with long term morbidity. RSV replication occurs primarily in the epithelium, producing a complex cellular response associated with acute inflammation and long-lived changes in pulmonary function and allergic disease. Proteomics approaches provide important insights into post-transcriptional regulatory processes including alterations in cellular complexes regulating the coordinated innate response and epigenome.

Areas covered: Peer-reviewed proteomics studies of host responses to RSV infections and proteomics techniques were analyzed. Methodologies identified include 1).” bottom-up” discovery proteomics, 2). Organellar proteomics by LC-gel fractionation; 3). Dynamic changes in protein interaction networks by LC-MS; and 4). selective reaction monitoring MS. We introduce recent developments in single-cell proteomics, top-down mass spectrometry, and photo-cleavable surfactant chemistries that will have impact on understanding how RSV induces extracellular matrix (ECM) composition and airway remodeling.

Expert opinion: RSV replication induces global changes in the cellular proteome, dynamic shifts in nuclear proteins, and remodeling of epigenetic regulatory complexes linked to the innate response. Pathways discovered by proteomics technologies have led to deeper mechanistic understanding of the roles of heat shock proteins, redox response, transcriptional elongation complex remodeling and ECM secretion remodeling in host responses to RSV infections and pathological sequelae.

摘要

简介：呼吸道合胞病毒（RSV）是一种与长期发病相关的主要人类病原体。RSV 复制主要发生在上皮细胞中，产生与急性炎症和肺功能长期变化和过敏性疾病相关的复杂细胞反应。蛋白质组学方法为转录后调控过程提供了重要的见解，包括调节协调先天反应和表观基因组的细胞复合物的改变。

涵盖的领域：对宿主对 RSV 感染的反应和蛋白质组学技术的同行评审蛋白质组学研究进行了分析。确定的方法包括 1)。自下而上”发现蛋白质组学，2）。通过 LC 凝胶分馏进行的细胞器蛋白质组学；3）。LC-MS 对蛋白质相互作用网络的动态变化；和 4)。选择性反应监测 MS。我们介绍了单细胞蛋白质组学、自上而下质谱法和光可裂解表面活性剂化学的最新进展，这些进展将对理解 RSV 如何诱导细胞外基质 (ECM) 组成和气道重塑产生影响。

专家意见：RSV 复制诱导细胞蛋白质组的整体变化、核蛋白的动态变化以及与先天反应相关的表观遗传调控复合物的重塑。蛋白质组学技术发现的途径使人们对热休克蛋白、氧化还原反应、转录延伸复合物重塑和 ECM 分泌重塑在宿主对 RSV 感染和病理后遗症的反应中的作用有了更深入的了解。