The usage of materials with the potential to accelerate wound healing is a great benefit for patients and health care systems. This study evaluated the impact of using graphene oxide (GO)–cellulose nanocomposite on skin wound healing via in vitro and in vivo investigations. The nanomaterial was synthesized and characterized. Cytocompatibility performance of the GO-cellulose was investigated through in vitro testing based on MTT and live/dead assays by EA.hy926 human endothelial cells (ECs). Additionally, the effect of GO-cellulose on induced wound scratch model using EA.hy926 ECs was investigated. Finally, the therapeutic effect of GO-cellulose was evaluated in vivo after the creation of two full-thickness wounds in the dorsum of rats (8 mm diameter). These wounds were randomly placed into two groups, the control group (10 wounds) and the GO-cellulose group (10 wounds), and monitored for gross and histopathological changes at 7 and 21 days after wound induction. MTT and Live/Dead assays showed excellent GO-cellulose cytocompatibility, whereas no difference in ECs viability was observed after culturing using conditioned media. GO-cellulose nanocomposite enhanced cell migration in the in vitro wound scratch assay. As compared to the control group, the GO-cellulose nanocomposite group's wound healing process was promoted in the in vivo rat skin wounds. Interestingly, wound re-epithelization and neovascularization were significantly accelerated in the GO-cellulose-treated rats. Furthermore, thick granulation tissue formation and intense collagen deposition were found in the GO-cellulose group. These findings showed that GO-cellulose has a promoting effect on skin wound healing, suggesting its promising and potential application in tissue regeneration.

使用具有加速伤口愈合潜能的材料对患者和医疗保健系统来说是一个巨大的好处。本研究评估使用氧化石墨烯（GO） -纤维素纳米复合材料上的皮肤伤口愈合的影响通过在体外和体内研究。合成并表征了纳米材料。通过基于MTT的体外测试和EA.hy926人内皮细胞（EC）的活/死分析，研究了GO纤维素的细胞相容性性能。另外，研究了使用EA.hy926 EC的GO纤维素对诱导的伤口刮擦模型的影响。最后，在体内评估了GO-纤维素的治疗效果在大鼠的背部（直径8毫米）中创建了两个全层伤口后。将这些伤口随机分为两组，即对照组（10个伤口）和GO-纤维素组（10个伤口），并在伤口诱导后第7天和第21天监测肉眼和组织病理学变化。MTT和活/死试验显示出优异的GO-纤维素细胞相容性，而在使用条件培养基培养后，未观察到EC活力的差异。GO-纤维素纳米复合材料在体外伤口刮擦试验中增强了细胞迁移。与对照组相比，GO-纤维素纳米复合材料组在体内的伤口愈合过程得到了促进大鼠皮肤伤口。有趣的是，在GO-纤维素治疗的大鼠中，伤口的再上皮形成和新生血管形成显着加速。此外，在GO-纤维素组中发现了厚实的肉芽组织形成和强烈的胶原蛋白沉积。这些发现表明GO-纤维素对皮肤伤口愈合具有促进作用，表明其在组织再生中有希望和潜在的应用。