ABSTRACT

The cellulose synthase superfamily is comprised of 36 genes in the cucumber genome database. The gene structure and motif analysis revealed that CsCLS genes are highly conserved. The gene ontology (GO) analysis of CsCLS genes unfolded their importance in growth-related and stress-responsive activities. The tissue-specific expression retrieved from RNA-seq data highlighted their importance as most of the CsCLS genes displayed expression in almost all of the tested tissues. Moreover, several genes showed upregulated expression patterns under the botanical fungicide (Chrysophanol and Physcion) treatment. The application of various abiotic (heat, cold, and NaCl stress) and biotic stresses (Powdery and Downy mildew) also stimulated the expression of CsCLS genes. The qRT-PCR analysis of CsCLS genes under gibberellic acid (GA) and melatonin (MT) presented upregulated expression trends in the majority of the genes, giving clues about their involvement in plant development and stress resistance. Furthermore, the transient expression of CsCSLS21 confirmed its location in the cell. Our result provides detailed knowledge of CsCLS genes in cucumber plants. We believe that this information will not only facilitate the development of stress-resilient plants but also improve their total biomass.

摘要

纤维素合酶超家族由黄瓜基因组数据库中的 36 个基因组成。基因结构和基序分析表明，CsCLS基因高度保守。CsCLS基因的基因本体 (GO) 分析揭示了它们在生长相关和应激反应活动中的重要性。从 RNA-seq 数据中检索到的组织特异性表达突出了它们的重要性，因为大多数CsCLS基因在几乎所有测试组织中都显示出表达。此外，在植物杀菌剂（Chrysophanol and Physcion）处理下，几个基因显示出上调的表达模式。各种非生物（热、冷和 NaCl 胁迫）和生物胁迫（白粉病和霜霉病）的应用也刺激了CsCLS基因。赤霉酸 (GA) 和褪黑激素 (MT) 下CsCLS基因的 qRT-PCR 分析显示了大多数基因的表达上调趋势，提供了有关它们参与植物发育和抗逆性的线索。此外，CsCSLS21的瞬时表达证实了它在细胞中的位置。我们的结果提供了黄瓜植物中CsCLS基因的详细知识。我们相信，这些信息不仅会促进抗逆植物的发展，还会提高它们的总生物量。