Aluminium is a non-essential metal and potentially toxic to organisms whose environmental concentration increases due to pollution. In our previous studies, the behavioral changes induced by aluminium were already shown on zebrafish, a model organism widely used for ecotoxicology screening. To examine in depth the knowledge about the toxicity mechanism induced by this metal, zebrafish embryos, at 6 hpf, have been exposed to 50, 100 and 200 µM of AlCl₃ for 72 h. Phenotypic alterations, apoptosis and oxidative stress responses have been assessed by evaluations of antioxidant defence and changes in metabolism at the end of treatment. The mRNA expression level of c-fos, appa and appb as marker genes of neural development and function were analyzed by qPCR for the highest used concentration. The data showed that aluminium significantly affected the development of zebrafish inducing morphological alterations and cell death. The oxidative state of larvae was altered, although the formation of reactive oxygen species and the levels of metallothioneins, and the activity of some antioxidant enzymes, decreased at the maximum concentration tested. In addition, at this concentration, the expression of the evaluated genes increased. The comprehensive information obtained gives a realistic snapshot of the aluminium toxicity and provides new information on the mechanism of action of this metal.

铝是一种非必需金属，对环境浓度因污染而增加的生物体具有潜在毒性。在我们之前的研究中，铝引起的行为变化已经在斑马鱼身上显示出来，斑马鱼是一种广泛用于生态毒理学筛选的模式生物。为了深入研究有关这种金属诱导的毒性机制的知识，6 hpf 的斑马鱼胚胎已暴露于 50、100 和 200 µM 的 AlCl ₃ 72 小时。表型改变、细胞凋亡和氧化应激反应已通过抗氧化防御和治疗结束时代谢变化的评估进行评估。c-fos、app a 和app的 mRNA 表达水平b 作为神经发育和功能的标记基因，通过 qPCR 分析最高使用浓度。数据显示铝显着影响斑马鱼的发育，诱导形态改变和细胞死亡。尽管活性氧的形成和金属硫蛋白的水平以及一些抗氧化酶的活性在测试的最大浓度下降低，但幼虫的氧化状态发生了改变。此外，在该浓度下，评估基因的表达增加。获得的综合信息给出了铝毒性的真实快照，并提供了有关这种金属作用机制的新信息。