Acellular Dermal Matrix (ADM) is mainly made with human or porcine skins and has the risk of zoonotic virus transmission. The fish skin-derived ADM could overcome the shortcoming. Fish skin acellular matrix has been used as wound dressing, but there is few systematic studies on tilapia-skin acellular dermal matrix (TS-ADM). In the present study, a novel TS-ADM was made by an alkaline decellularization process and γ-irradiation. The physical properties, biocompatibility, pre-clinical safety and wound healing activity of TS-ADM were systematically evaluated for its value as a functionally bioactive wound dressing. Histopathological analysis (hematoxylin and eosin staining, 4,6-diamidino-2-phenylindole (DAPI) staining) and DNA quantification both proved that the nuclear components of tilapia skin were removed sufficiently in TS-ADM. Compared to the commercial porcine acellular dermal matrix (DC-ADM), TS-ADM has distinctive features in morphology, thermal stability, degradability and water vapor transmission. TS-ADM was more readily degradable than DC-ADM in vitro and in vivo. In both rat and mini-pig skin wound healing experiments, TS-ADM was shown to significantly promote granulation growth, collagen deposition, angiogenesis and re-epithelialization, which may be attributed to the high expression of transforming growth factor-beta 1 (TGF-β1), alpha-smooth muscle actin (α-SMA) and CD31. Herein, the novel TS-ADM, used as a low-cost bioactive dressing, could form a microenvironment conducive to wound healing.

脱细胞真皮基质（ADM）主要由人皮或猪皮制成，具有传播人畜共患病毒的风险。鱼皮衍生的ADM可以克服这个缺点。鱼皮脱细胞基质已被用作伤口敷料，但对罗非鱼-皮肤脱细胞真皮基质（TS-ADM）的系统研究很少。在本研究中，一种新型 TS-ADM 通过碱性脱细胞过程和 γ 辐射制备。系统评估了 TS-ADM 作为功能性生物活性伤口敷料的物理特性、生物相容性、临床前安全性和伤口愈合活性。组织病理学分析（苏木精和伊红染色、4,6-二脒基-2-苯基吲哚（DAPI）染色）和 DNA 定量均证明罗非鱼皮肤的核成分在 TS-ADM 中被充分去除。与商品化猪脱细胞真皮基质 (DC-ADM) 相比，TS-ADM 在形态、热稳定性、降解性和水蒸气透过性方面具有鲜明的特点。在体外和体内，TS-ADM 比 DC-ADM 更容易降解。在大鼠和小型猪皮肤伤口愈合实验中，TS-ADM 均显示出显着促进肉芽生长、胶原沉积、血管生成和上皮再形成，这可能归因于转化生长因子-β1 (TGF- β1)、α-平滑肌肌动蛋白 (α-SMA) 和 CD31。在此，用作低成本生物活性敷料的新型 TS-ADM 可以形成有利于伤口愈合的微环境。在体外和体内，TS-ADM 比 DC-ADM 更容易降解。在大鼠和小型猪皮肤伤口愈合实验中，TS-ADM 均显示出显着促进肉芽生长、胶原沉积、血管生成和上皮再形成，这可能归因于转化生长因子-β1 (TGF- β1)、α-平滑肌肌动蛋白 (α-SMA) 和 CD31。在此，用作低成本生物活性敷料的新型 TS-ADM 可以形成有利于伤口愈合的微环境。在体外和体内，TS-ADM比DC-ADM更容易降解。在大鼠和小型猪皮肤伤口愈合实验中，TS-ADM被证明可显着促进肉芽生长，胶原蛋白沉积，血管生成和再上皮形成，这可能归因于转化生长因子-β1（TGF- β1)、α-平滑肌肌动蛋白 (α-SMA) 和 CD31。在此，用作低成本生物活性敷料的新型 TS-ADM 可以形成有利于伤口愈合的微环境。α-平滑肌肌动蛋白 (α-SMA) 和 CD31。在此，用作低成本生物活性敷料的新型 TS-ADM 可以形成有利于伤口愈合的微环境。α-平滑肌肌动蛋白（α-SMA）和CD31。在此，用作低成本生物活性敷料的新型 TS-ADM 可以形成有利于伤口愈合的微环境。