Nitric oxide (NO) produced in plant cells has the unique ability to interact with various other biomolecules, thereby facilitating its own as well as their signaling and associated actions at their sites of biosynthesis and at other sites via transcellular long distance transport of the molecular complexes. Melatonin (Mel) is one such biomolecule produced in plant cells which has fascinated plant biologists with regard to its molecular crosstalk with other molecules to serve its roles as a growth regulator. Present work reports the synthesis of N-nitrosomelatonin (NOMela) and its preferential uptake by Arabidopsis seedlings roots and long distance transport to the leaves through vascular strands. Equimolar (250 μM) concentrations of NOMela and S-nitrosoglutathione (GSNO) in aqueous solutions bring about 52.8% more release of NO from NOMela than from GSNO. Following confocal laser scanning microscopic (CLSM) imaging, Pearson's correlation coefficient analysis of the Scatter gram of endogenously taken up NOMela demonstrates significant NO signal in roots emanating from mitochondria. NOMela (250 μM) taken up by Arabidopsis seedling roots also proved more efficient as a NO transporter from primary root to leaves than 250 μM of GSNO. These novel observations on NOMela thus hold promise to decipher its crucial role as a NO carrier and reservoir in plant cells, and also as a facilitator of melatonin action in plant development.

植物细胞中产生的一氧化氮 (NO) 具有与各种其他生物分子相互作用的独特能力，从而通过分子复合物的跨细胞长距离运输在其生物合成位点和其他位点促进其自身以及它们的信号传导和相关作用. 褪黑激素 (Mel) 是一种在植物细胞中产生的此类生物分子，植物生物学家因其与其他分子的分子串扰以发挥其作为生长调节剂的作用而着迷。目前的工作报告了N的合成-亚硝基褪黑激素（NOMela）及其被拟南芥幼苗根部优先吸收并通过维管束长距离运输至叶子。水溶液中等摩尔 (250 μM) 浓度的 NOMela 和 S-亚硝基谷胱甘肽 (GSNO) 使 NOMela 释放的 NO 比 GSNO 多 52.8%。在共聚焦激光扫描显微 (CLSM) 成像后，Pearson 对内源性 NOMela 的 Scatter 克的相关系数分析表明，线粒体发出的根中有显着的 NO 信号。与 250 μM 的 GSNO 相比，拟南芥幼苗根部吸收的 NOMela (250 μM) 作为 NO 转运蛋白从初生根到叶子的效率更高。因此，这些关于 NOMela 的新观察有望破译其在植物细胞中作为 NO 载体和储库的关键作用，