Diazepam (DZP) is a benzodiazepine drug used as an anti-drug and sedative. It is often misused to induce or create euphoria in combination with other drugs (high or fly sensation) or administered alone. So far, screening for DZP abuse with sensitive analytical methods is needed, as its small concentrations make it difficult to detect. Increased sensitivity of the analytical method can be obtained by using a preparation method that selectively separates the analyte from the sample matrix. Molecularly imprinted polymer (MIP) is one of the preparation solutions with good selectivity, specificity, and sensitivity. MIP was made from DZP as a template, itaconic acid, and ethylene glycol dimethacrylate in a composition of 1:4:20. MIP was made by precipitation polymerisation to obtain microsphere polymer type. MIP had a binding capacity value of 0.0557 mg/g and followed the Freundlich isotherm. Application of the microsphere MIP on spiked blood serum resulted in a recovery of 105.63 ± 1.0% for MIP compared to 21.28 ± 0.4% for non-imprinted polymer, with the imprinting factor value reaching 4.96. Hence, MIP DZP with itaconic acid as a functional monomer and propanol as a porogen, fabricated by the precipitation polymerisation method, is a promising sorbent for DZP extraction in biological fluids.

中文翻译：

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使用衣康酸作为丙醇中的单体的微球分子印迹固相萃取法进行地西analysis分析

地西p（DZP）是用作抗药和镇静剂的苯二氮卓类药物。通常与其他药物（强烈或苍蝇感）结合使用或单独使用时，会被误用为诱发或产生欣快感。到目前为止，由于DZP的浓度很小，难以检测，因此需要使用灵敏的分析方法进行DZP滥用的筛查。通过使用选择性地从样品基质中分离出分析物的制备方法，可以提高分析方法的灵敏度。分子印迹聚合物（MIP）是具有良好的选择性，特异性和敏感性的制备溶液之一。MIP由DZP作为模板，衣康酸和乙二醇二甲基丙烯酸酯以1：4：20的组成制成。通过沉淀聚合制备MIP以获得微球聚合物类型。MIP的绑定能力值为0。0557 mg / g，然后遵循Freundlich等温线。将微球MIP应用于加标血清后，MIP的回收率为105.63±1.0％，而非印迹聚合物的回收率为21.28±0.4％，印迹因子值达到4.96。因此，通过沉淀聚合法制备的以衣康酸为功能单体，丙醇为致孔剂的MIP DZP是一种有前途的吸附剂，可用于生物流体中DZP的提取。