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Development of 79 SNP markers to individually genotype and sex-type endangered mountain gorillas ( Gorilla beringei beringei )
Conservation Genetics Resources ( IF 1.1 ) Pub Date : 2021-05-20 , DOI: 10.1007/s12686-021-01217-4
Benjamin N. Sacks , Tianyi Hu , Elizabeth M. Kierepka , Stevi L. Vanderzwan , Jena R. Hickey

The mountain gorilla (Gorilla beringei beringei) is one of two endangered subspecies of eastern gorilla. The principle approach to monitoring the two extant mountain gorilla populations has been to use fecal surveys to obtain DNA profiles for individuals that are then used for capture-recapture-based estimates of abundance. To date, 11 to 14 microsatellites have been used for this purpose. To adapt to ongoing changes in genotyping technologies and to facilitate the analysis of fecal DNA samples by multiple laboratories, we developed a panel of single nucleotide polymorphism (SNP) markers that can be used for future gorilla monitoring. We used published short read data sets for 3 individuals to develop a suite of 79 SNPs, including two sex markers, for a Fluidigm platform. This marker set provided high resolution to differentiate individuals and will facilitate future monitoring, leaving room for additional SNPs to be included in a 96-assay format.



中文翻译:

开发79个SNP标记分别针对个别基因型和性别类型的濒临灭绝的山地大猩猩(Gorilla beringei beringei)

大猩猩山(大猩猩beringei beringei)是东部大猩猩的两个濒危亚种之一。监测两个现存山地大猩猩种群的主要方法是使用粪便调查获得个体的DNA概况,然后将其用于基于捕获-捕获的丰度估计。迄今为止,已为此目的使用了11至14个微卫星。为了适应基因分型技术的不断变化并促进多个实验室对粪便DNA样品的分析,我们开发了一组单核苷酸多态性(SNP)标记,可用于将来的大猩猩监测。我们使用已发布的3个个体的简短阅读数据集,为Fluidigm平台开发了一套79个SNP的套件,其中包括两个性别标记。此标记集可提供高分辨率以区分个人,并有助于将来进行监控,

更新日期:2021-05-20
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