Coriolus versicolor is a well-known edible and medicinal fungus containing polysaccharides and polysaccharopeptides, which have been proven to possess interesting bioactivities, especially antitumor and immunomodulatory activities. A facile and sensitive method for the monosaccharide composition analysis of polysaccharides in C. versicolor has been developed by employing precolumn derivatization ultra-performance liquid chromatography equipped with a photodiode array detector (UPLC-PDA). The hydrolysis conditions of polysaccharides from C. versicolor and the optimization of the sample precolumn derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) were comprehensively studied. The LODs of the quantity of monosaccharides were in the range of 3.1–4.6 ng. The PMP-labeled mixture of monosaccharides could be successfully separated by UPLC and detected by a PDA detector under optimized conditions. The mobile phase of the elution system was chosen as acetonitrile and 25 mm K₂HPO₄ (pH 6.5) with the Phenomenex Kinetex C18 column, and the column temperature was set at 35 °C. The C. versicolor polysaccharides mainly consist of mannose (Man), glucose (Glc), galactose (Gal), fucose (Fuc), trace rhamnose (Rha), ribose (Rib), glucuronic acid (GlcA), galacturonic acid (GalA), xylose (Xyl), and arabinose (Ara), and the content of Glc is the highest.

云芝是一种众所周知的食用和药用真菌，含有多糖和多糖肽，已被证明具有有趣的生物活性，尤其是抗肿瘤和免疫调节活性。通过采用配备光电二极管阵列检测器（UPLC-PDA）的柱前衍生超高效液相色谱法，已经开发了一种简便而灵敏的方法，用于分析云芝中多糖的单糖成分。云芝多糖的水解条件全面研究了用1-苯基-3-甲基-5-吡唑啉酮（PMP）进行样品前柱衍生化的优化方法。单糖含量的LOD在3.1–4.6 ng范围内。PMP标记的单糖混合物可通过UPLC成功分离，并在最佳条件下用PDA检测器检测。使用Phenomenex Kinetex C18色谱柱，将洗脱系统的流动相选择为乙腈和25 mm K ₂ HPO ₄（pH 6.5），并将色谱柱温度设定为35°C。该云芝 多糖主要包括甘露糖（Man），葡萄糖（Glc），半乳糖（Gal），岩藻糖（Fuc），微量鼠李糖（Rha），核糖（Rib），葡萄糖醛酸（GlcA），半乳糖醛酸（GalA），木糖（Xyl ）和阿拉伯糖（Ara），且Glc的含量最高。