Medicinal plants are used as an important source of medicines in pharmaceutical industry. Rubia cordifolia is widely used to cure diabetes mellitus. Present study was aimed to investigate the antihyperglycemic effects of different fractions of R. cordifolia leaves and to analyze its antioxidant effect and phytochemical composition. Male albino mice were randomly distributed into seven groups ( n = 7). Group-I was normal control, group-II was Alloxan (100 mg/kg)-induced diabetic control, and group-III was standard drug (Glibenclamide 0.5 mg/kg)-treated group. Animals in groups IV–VII were treated with n -hexane fraction, ethyl acetate fraction, n -butanol fraction and aqueous fraction of R. cordifolia , orally administered (100 mg/kg) once daily up to 28 days after Alloxan induction, respectively. Methanolic extract (ME) and fractions of R. cordifilia were analyzed for antioxidant activity and quantification of total phenolic content and total flavonoid content. HPLC of ME and most active fractions were performed. The results showed that RCEF (G-V) and RCBF (G-VI) have significantly ( P < 0.05) reduced the increased level of glucose as compared to toxicant control group. It was further revealed that EF and BF have higher antioxidant activity (having IC 50 34.9, 36.86 (µg/mL)) owing to phenolic and flavonoid identified by HPLC.

中文翻译：

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茜草叶提取物的降血糖作用及植物化学研究

药用植物被用作制药工业中重要的药物来源。茜草被广泛用于治疗糖尿病。本研究旨在研究不同等级的串叶枫叶的降血糖作用，并分析其抗氧化作用和植物化学成分。将雄性白化病小鼠随机分为7组（n = 7）。第一组是正常对照组，第二组是四氧嘧啶（100 mg / kg）诱导的糖尿病对照组，第三组是标准药物（格列本脲0.5 mg / kg）治疗组。IV-VII组的动物分别接受正己烷级分，乙酸乙酯级分，正丁醇级分和Cordifolia的水性级分处理，分别在四氧嘧啶诱导后直至28天每天口服（100 mg / kg）。甲醇提取物（ME）和R的馏分 分析了紫的抗氧化活性，并对总酚含量和总黄酮含量进行了定量。进行了ME和大多数活性馏分的HPLC。结果表明，与有毒对照组相比，RCEF（GV）和RCBF（G-VI）显着（P <0.05）降低了葡萄糖的升高水平。进一步表明，由于通过HPLC鉴定出的酚和类黄酮，EF和BF具有较高的抗氧化活性（IC 50为34.9，36.86（µg / mL））。