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Electrical therapies act on the Ca2+/CaM signaling pathway to enhance bone regeneration with bioactive glass [S53P4] and allogeneic grafts
Journal of Biomedical Materials Research Part B: Applied Biomaterials ( IF 3.4 ) Pub Date : 2021-05-18 , DOI: 10.1002/jbm.b.34858
Leonardo Bagne 1 , Maraiara A Oliveira 1 , Amanda T Pereira 1 , Guilherme F Caetano 1 , Camila A Oliveira 1 , Andréa A Aro 1 , Gabriela B Chiarotto 1 , Glaucia M T Santos 1 , Fernanda A S Mendonça 1 , Milton Santamaria-Jr 1
Affiliation  

This study aimed to investigate the application of low-intensity electrostimulation (ES) and electromagnetic stimulation (EM) associated with bioactive glass (BG) or allogeneic grafts (BB) in bone regeneration. A cell viability test on osteoblasts (UMR-106) was performed in the presence of BB and BG grafts associated with ES (10 μA/5 min) and EM (500 Hz/2 min). Critical defects (25 mm2) in calvaria were generated in male Wistar rats, and bone regeneration was evaluated on the 30th, 60th, and 120th days after surgery. Cell proliferation increased with the application of ES in both grafts and after EM with BG. Bone remodeling was more effective using the allogeneic graft in both therapies, with increased angiogenesis, osteoblast proliferation, and OPN expression in the BB + EM group. A higher number of osteoblasts and osteoclasts, and an increase in bone sialoprotein, Runx-2, and Opn gene expression were found in the BB + ES group. The BG graft associated with EM therapy had an increased proliferation of osteoblasts and increased expression of Runx-2 and Opn. Groups that had BG and ES therapy had increased numbers of osteoblasts, osteoclasts, and increased OPN expression. The expression of voltage-gated calcium channels increased in groups with ES, while calmodulin expression increased in therapies without grafting. ES and EM therapies favored the repair of bone defects upon grafting by improving angiogenesis, osteogenic gene expression, and tissue reorganization. Despite activating different pathways, both therapies increased the intracellular concentrations of calmodulin, leading to cell proliferation and bone regeneration.

中文翻译:

电疗法作用于 Ca2+/CaM 信号通路,通过生物活性玻璃 [S53P4] 和同种异体移植物增强骨再生

本研究旨在探讨与生物活性玻璃 (BG) 或同种异体移植物 (BB) 相关的低强度电刺激 (ES) 和电磁刺激 (EM) 在骨再生中的应用。在存在与 ES (10 μA/5 min) 和 EM (500 Hz/2 min) 相关的 BB 和 BG 移植物的情况下,对成骨细胞 (UMR-106) 进行细胞活力测试。雄性 Wistar 大鼠颅骨严重缺损 (25 mm 2 ),在术后第 30、60 和 120 天评估骨再生。细胞增殖随着在移植物中应用 ES 和在 EM 与 BG 后的应用而增加。在两种疗法中使用同种异体移植物的骨重塑更有效,血管生成、成骨细胞增殖和OPN增加BB + EM 组中的表达。在 BB + ES 组中发现更多的成骨细胞和破骨细胞,以及骨唾液蛋白、Runx-2Opn基因表达增加。与 EM 治疗相关的 BG 移植物具有增加的成骨细胞增殖和增加的Runx-2Opn表达。接受 BG 和 ES 治疗的组的成骨细胞、破骨细胞数量增加,OPN增加表达。ES 组电压门控钙通道的表达增加,而没有移植的治疗组钙调蛋白表达增加。ES 和 EM 疗法有利于通过改善血管生成、成骨基因表达和组织重组来修复移植后的骨缺损。尽管激活了不同的途径,两种疗法都增加了细胞内钙调蛋白的浓度,导致细胞增殖和骨再生。
更新日期:2021-05-18
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