Biochemical Genetics ( IF 2.4 ) Pub Date : 2021-05-19 , DOI: 10.1007/s10528-021-10077-4 Meiying Li 1, 2 , Licheng Ren 3 , Zhi Zou 1, 2 , Wei Hu 1, 2 , Susheng Xiao 1, 2 , Xiaoliang Yang 1, 2 , Zehong Ding 1, 2 , Yan Yan 1, 2 , Weiwei Tie 1, 2 , Jinghao Yang 1, 2 , Anping Guo 1, 2
Plant 14–3-3 proteins play key roles in regulating growth, development, and stress responses. However, little is known about this gene family in papaya (Carica papaya L.). We characterized eight 14–3-3 genes from the papaya genome and designed them as CpGRF1–8. Based on phylogenetic, conserved motif, and gene structure analyses, papaya CpGRFs were divided into ε and non-ε groups. Expression analysis showed differential and class-specific transcription patterns in different organs. Quantitative real-time polymerase chain reaction analysis showed that most CpGRFs had large changes in expression during fruit development and ripening. This indicated that the CpGRFs were involved in regulating fruit development and ripening. Significant expression changes occurred after cold, salt, and drought treatments in papaya seedlings, indicating that CpGRFs were also involved in signaling responses to abiotic stress. These results provide a transcription profile of 14–3-3 genes in organs, during fruit development and ripening and in response to stress. Some highly expressed, fruit-specific, and stress-responsive candidate CpGRFs will be identified for further genetic improvement of papayas.
中文翻译:
木瓜特殊 14-3-3 基因家族的鉴定和表达分析及其在果实发育、成熟和非生物胁迫反应中的作用
植物 14-3-3 蛋白在调节生长、发育和应激反应中起关键作用。然而,人们对木瓜( Carica papaya L.)中的这个基因家族知之甚少。我们对木瓜基因组中的 8 个14-3-3基因进行了表征,并将它们设计为CpGRF1-8。基于系统发育、保守基序和基因结构分析,木瓜CpGRF分为 ε 组和非 ε 组。表达分析显示不同器官中的差异和类特异性转录模式。定量实时聚合酶链反应分析表明,大多数CpGRFs在果实发育和成熟过程中的表达变化很大。这表明CpGRF参与调节果实发育和成熟。木瓜幼苗在冷、盐和干旱处理后发生了显着的表达变化,表明CpGRFs也参与了对非生物胁迫的信号反应。这些结果提供了器官中14-3-3基因的转录谱,在果实发育和成熟期间以及对压力的反应。将确定一些高度表达、水果特异性和应激反应的候选CpGRF,用于进一步改良木瓜的遗传。