Abstract

Diabetic wounds are very problematic wounds that have a high risk of infection. The healing process of wounds in diabetic patients is more complicated than others. Wound dressing preparation is one of the promising treatment modalities for repairing damaged tissues in diabetic patients. The aim of this study is to demonstrate the antibacterial effects of drug release of clarithromycin from poly(lactic acid) (PLA) nanofibers incorporated with selenium (Se) and graphene-oxide (GO) to reveal their wound healing potential. In the present study, optimized PLA was combined with graphene oxide (GO) with a concentration of 0.45 wt%, 1 wt%, 1.50 wt%, 2 wt%, 2.50 wt%, and 3 wt%, respectively, and the combination was produced by modified Hummers method. After that, optimized PLA/GO was incorporated with Se and clarithromycin with PLA with the percentage by weight of 1:1, nanofiber patches were successfully produced by the electrospinning technique. In vitro antibiotic test, cell culture tests (cytotoxicity test, cell adhesion, and in vitro wound healing assay), morphological analysis (SEM), molecular interactions between the components (FT-IR), tensile strength, and physical analysis (viscosity, surface tensile, density and conductivity tests) were carried out after production of nanofiber patches. According to the results, the average pore size of 8 wt% PLA + 1 wt% GO + 1 wt% Se + 1 wt% clarithromycin nanofiber is 640 nm. Moreover, 8 wt% PLA + 1 wt% GO + 1 wt% Se + 1 wt% clarithromycin has the highest viscosity and surface tension value than others with the values of 253.8 ± 6.67 mPas and 31.62 ± 2.13 mN/m, respectively. Finally, it was observed that Se-incorporated electrospun nanofibers had antibacterial effects and are highly promising wound healing materials. To sum up, the prepared nanofibers illustrated important suitable mechanical properties, controlled release and antibacterial effect and results showed that Se incorporated PLA-GO-drug composite is a promising wound healing material.

摘要

糖尿病伤口是非常有问题的伤口，具有很高的感染风险。糖尿病患者的伤口愈合过程比其他人更复杂。伤口敷料制备是修复糖尿病患者受损组织的有前途的治疗方式之一。本研究的目的是证明含有硒 (Se) 和氧化石墨烯 (GO) 的聚乳酸 (PLA) 纳米纤维释放克拉霉素药物的抗菌作用，以揭示其伤口愈合潜力。在本研究中，优化的 PLA 与氧化石墨烯 (GO) 的浓度分别为 0.45 wt%、1 wt%、1.50 wt%、2 wt%、2.50 wt% 和 3 wt%，组合物为采用改良悍马法生产。在那之后，体外抗生素试验、细胞培养试验（细胞毒性试验、细胞粘附试验、体外在纳米纤维贴片生产后进行了伤口愈合试验）、形态分析（SEM）、组分之间的分子相互作用（FT-IR）、拉伸强度和物理分析（粘度、表面拉伸、密度和电导率测试）。根据结果​​，8 wt% PLA + 1 wt% GO + 1 wt% Se + 1 wt% 克拉霉素纳米纤维的平均孔径为640 nm。此外，8 wt% PLA + 1 wt% GO + 1 wt% Se + 1 wt% 克拉霉素具有最高的粘度和表面张力值，分别为 253.8 ± 6.67 mPas 和 31.62 ± 2.13 mN/m。最后，观察到掺硒的电纺纳米纤维具有抗菌作用，是非常有前景的伤口愈合材料。综上所述，制备的纳米纤维展示了重要的合适的机械性能，