The purpose of this study was to develop a method for the simultaneous qualitative and quantitative analysis of ursolic acid (UA) and oleanolic acid (OA) in Punica granatum L. flowers. UA and OA were analyzed qualitatively and quantitatively by high-performance thin-layer chromatography (HPTLC). The thin layers chosen were high-performance thin-layer G plates. Sample spots were pretreated in situ with iodine–dichloromethane solution with cyclohexane–ethyl acetate–methanol (8.2:1.8:0.5, V/V) as developing agent upward. The plates were immersed in a 1% sulphuric acid–ethanol solution, dried and heated at 105 °C for 30 min to develop the color and then it was scanned at the wavelength of 530 nm. The results revealed that the R_F values for UA and OA were 0.43 ± 0.05 and 0.62 ± 0.05, respectively, with a linear range of 0.2595–3.633 μg and 0.2505–1.503 μg, with correlation coefficients of 0.9980 and 0.9954, representing a sound linear relationship. The limits of detection (LOD) for UA and OA were 10 and 20 ng, with their limits of quantification (LOQ) of 33 and 67 ng, respectively. Average recoveries of 101.38% and 100.13% were obtained with relative standard deviations (RSD) of 2.55% and 2.74%. In summary, it can be concluded that UA and OA in P. granatum L. flowers could be completely separated by in situ pretreatment, and this chromatographic method could quickly and accurately determine the content of UA and OA in P. granatum L. flowers.

本研究的目的是开发一种用于在熊果酸（UA）和齐墩果酸（OA）的同时定性，定量分析的方法石榴L.花。通过高效薄层色谱法（HPTLC）对UA和OA进行定性和定量分析。选择的薄层是高性能薄层G板。样品斑点用碘-二氯甲烷溶液原位预处理，其中环己烷-乙酸乙酯-甲醇（8.2：1.8：0.5，V / V）作为显影剂向上处理。将板浸入1％的硫酸-乙醇溶液中，干燥并在105°C加热30分钟以显色，然后在530 nm的波长下进行扫描。结果表明，R _FUA和OA的数值分别为0.43±0.05和0.62±0.05，线性范围为0.2595–3.633μg和0.2505–1.503μg，相关系数为0.9980和0.9954，代表了良好的线性关系。UA和OA的检出限（LOD）分别为10和20 ng，定量限（LOQ）分别为33和67 ng。获得的平均回收率为101.38％和100.13％，相对标准偏差（RSD）为2.55％和2.74％。总之，可以得出结论，原位预处理可以完全分离颗粒状花中UA和OA ，该色谱方法可以快速，准确地测定颗粒状花中UA和OA的含量。