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Isolation and identification of an osmotolerant Bacillus amyloliquefaciens strain T4 for 2, 3-butanediol production with tobacco waste
Preparative Biochemistry & Biotechnology ( IF 2.9 ) Pub Date : 2021-05-19 , DOI: 10.1080/10826068.2021.1925912
Ju Han 1, 2 , Fan Wang 1, 2 , Zhihao Li 1, 3 , Lijuan Liu 1 , Ge Zhang 1, 2 , Guoqiang Chen 1, 2 , Jing Liu 4 , Haibo Zhang 1, 2
Affiliation  

Abstract

Microbial biomass and waste materials conversion for biochemicals production has been an alternative for energy conservation and emission reduction. While toxic substances in biomass materials and high osmotic pressure formed in fermentation-based systems block the bioconversion processes of microorganisms. In the present study, strain T4 that isolated from tobacco waste could resist toxic inhibitors such as nicotine and was suitable for generation of 2, 3-butanediol (2, 3-BD) with a high concentration of glucose (up to 20%). 30.06 and 1.54 g/L of 2, 3-BD was generated respectively from 50 g/L of tobacco waste with and without 200 g/L glucose after fermentation for 48 h. Besides, the results of biochemical tests showed that it was gram-positive and able to liquefy gelatin, hydrolyze starch and produce catalases. It could utilize glucose but not lactose as carbohydrates during fermentation. The 16S rRNA sequence and systematic analysis revealed that T4 was identified to be a Bacillus amyloliquefaciens (B. amyloliquefaciens). This work presents a promising model microorganism chassis to use the biomass waste for high value-added biochemicals production.



中文翻译:

用于烟草废料生产 2, 3-丁二醇的渗透性解淀粉芽孢杆菌 T4 菌株的分离和鉴定

摘要

用于生化生产的微生物生物质和废料转化已成为节能减排的替代方案。而生物质材料中的有毒物质和发酵系统中形成的高渗透压阻碍了微生物的生物转化过程。在本研究中,从烟草废料中分离得到的 T4 菌株可以抵抗尼古丁等有毒抑制剂,适合生成高浓度葡萄糖(高达 20%)的 2, 3-丁二醇(2, 3-BD)。50 g/L 的烟草废料在发酵 48 h 后加入和不加入 200 g/L 葡萄糖分别产生 30.06 和 1.54 g/L 的 2, 3-BD。此外,生化试验结果表明,它是革兰氏阳性菌,能够液化明胶、水解淀粉和产生过氧化氢酶。它可以在发酵过程中利用葡萄糖而不是乳糖作为碳水化合物。16S rRNA 序列和系统分析表明,T4 被鉴定为解淀粉芽孢杆菌B. amyloliquefaciens)。这项工作提出了一种有前景的模型微生物底盘,可将生物质废物用于高附加值生化产品的生产。

更新日期:2021-05-19
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