ABSTRACT

Background

Entamoeba gingivalis has been associated with periodontal diseases. Baseline data from the background population, which could help delimit the role of the parasite in health and disease, remain limited.

Objective

To describe epidemiological features, genetic diversity, and associations with oral microbiome signatures of E. gingivalis colonisation in Tanzanians with non-oral/non-dental diseases.

Methods

DNAs from 92 oral washings from 52 participants were subject to metabarcoding of ribosomal genes. DNA sequences were identified to genus level and submitted to oral microbiota diversity analyses.

Results

Sixteen (31%) of the 52 study participants were E. gingivalis-positive, with no difference in positivity rate according to gender or age. Only one subtype (ST1) was found. Individuals testing positive for E. gingivalis had higher oral microbiota alpha diversity than those testing negative (P = 0.03). Eight of the top-ten most common bacterial genera were shared between the two groups (Alloprevotella, Fusobacterium, Gemella, Haemophilus, Neisseria, Porphyromonas, Prevotella, Streptococcus, and Veillonella). Meanwhile, E. gingivalis carriers and non-carriers were more likely to have Aggregatibacter and Rothia, respectively, among the top-ten most common genera.

Conclusion

About one third of the cohort carried E. gingivalis ST1, and carriers had higher oral microbiome diversity and were more predominantly colonized by Aggregatibacter.

中文翻译：

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牙龈内阿米巴：坦桑尼亚东北部的流行病学、遗传多样性和与口腔微生物群特征的关联

摘要

背景

牙龈内阿米巴与牙周病有关。来自背景人群的基线数据可能有助于界定寄生虫在健康和疾病中的作用，但仍然有限。

客观的

描述坦桑尼亚非口腔/非牙科疾病牙龈大肠杆菌定植的流行病学特征、遗传多样性以及与口腔微生物组特征的关联。

方法

来自 52 名参与者的 92 次口腔清洗液的 DNA 接受了核糖体基因的元条形码编码。DNA序列被鉴定到属水平并提交给口腔微生物群多样性分析。

结果

52 名研究参与者中有 16 名 (31%) 为E. gingivalis阳性，阳性率无性别或年龄差异。仅发现一种亚型（ST1）。E. gingivalis检测呈阳性的个体口腔微生物群 alpha 多样性高于检测呈阴性的个体（P = 0.03）。两组最常见的十个细菌属中的八个属（别氏菌属、梭杆菌属、双菌属、嗜血杆菌属、奈瑟氏菌属、卟啉单胞菌属、普氏菌属、链球菌属和韦荣氏菌属）。同时，E. gingivalis携带者和非携带者更有可能感染Aggregatibacter和Rothia，分别位居前十名最常见的属。

结论

大约三分之一的队列携带E. gingivalis ST1，携带者具有更高的口腔微生物群多样性，并且更主要地被Aggregatibacter定植。