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Green and Cost-Effective Extraction Techniques of Quercetin from Mixture of Nutraceuticals with Yield Analysis via Spectrophotometry and High-Performance Liquid Chromatography Methods
Journal of AOAC INTERNATIONAL ( IF 1.6 ) Pub Date : 2021-05-07 , DOI: 10.1093/jaoacint/qsab071
Mohammed Gamal 1, 2 , Heba-Alla H Abd-ElSalam 3 , Ibrahim A Naguib 4 , Medhat A Al-Ghobashy 5 , Hala E Zaazaa 5 , M Abdelkawy 6
Affiliation  

Background Extraction is the leading critical stage in the analysis of nutraceuticals. Ginkgo biloba (GB) has gained interest because of its therapeutic usages. Objectives The aim was to develop four cost-effective extraction techniques for the extraction of quercetin from GB in a sachet containing a mixture of nutraceuticals. These techniques are solid-phase extraction (SPE), liquid–liquid extraction, inverted dispersive liquid–liquid microextraction, and the QuEChERS (quick, easy, cheap, effective, rugged, and safe) method. Method Direct spectrophotometry was used to monitor the recovery of the standard quercetin throughout the optimization steps. The HPLC–UV method of analysis was optimized to quantify the yields from the extracts present in the complicated contents of the sachets. The present study was assessed by analytical Eco-Scale assessment (ESA) and the National Environmental Method Index (NEMI) for greenness in comparison with the literature. Results SPE showed the best cleanup outcomes. ESA and NEMI showed an adequate greenness of the proposed extraction protocol. Conclusions Quercetin (marker for GB) extraction from market nutraceutical sachets is considered an exemplar for analysis in the QC of nutraceuticals. Regarding the greenness results, the proposed method of extraction is better even with adequate greenness as the extraction was a one-step process, in comparison with multistep processes of previously published protocols. Accordingly, it is recommended for use in routine extraction and analysis of such nutraceuticals. Highlights Four extraction protocols have been developed. For GB ternary-mixture sachets, proper recovery was obtained using C18 SPE. The assessment of greenness of the proposed protocol guaranteed the superiority of the presented method. Safer sorbents and chemicals are favored for use in routine extraction of nutraceuticals.

中文翻译:

通过分光光度法和高效液相色谱法从保健品混合物中提取槲皮素的绿色和经济高效的提取技术

背景提取是营养保健品分析中的主要关键阶段。银杏叶 (GB) 因其治疗用途而受到关注。目的 目的是开发四种具有成本效益的提取技术,用于从含有营养保健品混合物的小袋中提取 GB 中的槲皮素。这些技术是固相萃取 (SPE)、液-液萃取、反向分散液-液微萃取和 QuEChERS(快速、简单、便宜、有效、耐用和安全)方法。方法 直接分光光度法用于监测整个优化步骤中标准槲皮素的回收率。优化了 HPLC-UV 分析方法,以量化小袋复杂内容物中提取物的产量。本研究通过分析生态尺度评估 (ESA) 和国家环境方法指数 (NEMI) 与文献进行比较来评估绿色。结果 SPE 显示出最好的净化效果。ESA 和 NEMI 表明所提出的提取方案具有足够的绿色性。结论 从市售的保健食品袋中提取槲皮素(GB 标志物)被认为是保健食品 QC 分析的范例。关于绿色结果,与先前发布的协议的多步过程相比,即使具有足够的绿色,所提出的提取方法也更好,因为提取是一步过程。因此,建议将其用于此类保健品的常规提取和分析。亮点 已经开发了四种提取协议。对于国标三元混合小袋,使用 C18 SPE 获得了适当的回收率。对拟议协议的绿色评估保证了所提出方法的优越性。更安全的吸附剂和化学品更适合用于营养保健品的常规提取。
更新日期:2021-05-07
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