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Ammonium transporter PsAMT1.2 from Populus simonii functions in nitrogen uptake and salt resistance
Tree Physiology ( IF 4 ) Pub Date : 2021-05-17 , DOI: 10.1093/treephys/tpab071
Wenxin Li 1, 2 , Zimao Feng 2 , Chunxia Zhang 1, 2
Affiliation  

Ammonium (NH4+) is a primary nitrogen (N) source for many species, and NH4+ uptake is mediated by various transporters. However, the effects of NH4+ transporters on N uptake and metabolism under salt stress, remain unclear. In the present study, we investigated the expression characteristics and transport function of PsAMT1.2 in Populus simonii and its role in ammonium uptake and metabolism under salt stress. PsAMT1.2 was localized in the plasma membrane and was highly expressed in the roots. Heterologous functionality tests demonstrated that PsAMT1.2 mediates NH4+ permeation across the plasma membrane in yeast mutants, restoring growth. A short-term NH4+ uptake experiment showed that PsAMT1.2 is a high-affinity NH4+ transporter with a Km value of 80.603 μM for NH4+. Compared with the wild type (WT), PsAMT1.2-overexpressing transgenic poplar grew better, with higher increases in stem height and relative chlorophyll content under both control and salt-stress conditions. PsAMT1.2 significantly increased the total NH4+ concentration and total N of whole plants under salt stress. The glutamate synthase (GS), glutamine synthetase (GOGAT), and glutamate dehydrogenase (GDH) activities and the total amino acids largely increased in the roots of PsAMT1.2-overexpressing transgenic plants compared with the WT plants under control conditions, suggesting that PsAMT1.2 promotes NH4+ assimilation and metabolism in poplar roots. Consistent with the increased total amino acid content, GS1.3, GS2, and Fd-GOGAT expression was upregulated in the roots and leaves of the PsAMT1.2-overexpressing transgenic plants compared with the WT plants under salt stress. Collectively, PsAMT1.2 encodes a high-affinity NH4+ transporter crucial to NH4+ uptake and metabolism under salt stress.

中文翻译:

来自小杨的铵转运蛋白 PsAMT1.2 在氮吸收和耐盐性中发挥作用

铵 (NH 4 + ) 是许多物种的主要氮 (N) 来源,并且 NH 4 +的吸收由各种转运蛋白介导。然而,盐胁迫下NH 4 +转运蛋白对N 吸收和代谢的影响仍不清楚。在本研究中,我们研究了PsAMT1.2小叶杨中的表达特征和转运功能及其在盐胁迫下铵吸收和代谢中的作用。PsAMT1.2定位于质膜并在根中高度表达。异源功能测试表明PsAMT1.2介导 NH 4 +穿透酵母突变体的质膜,恢复生长。短期NH 4 +吸收实验表明,PsAMT1.2是一种高亲和力的NH 4 +转运蛋白,对NH 4 +的Km值为80.603 μM 。与野生型(WT)相比,过表达PsAMT1.2的转基因杨树生长更好,在对照和盐胁迫条件下,茎高和相对叶绿素含量增加更高。PsAMT1.2显着增加总NH 4 +盐胁迫下整株植物的浓度和总氮含量。与对照条件下的 WT 植物相比,过表达PsAMT1.2的转基因植物根中的谷氨酸合成酶 (GS)、谷氨酰胺合成酶 (GOGAT) 和谷氨酸脱氢酶 (GDH) 活性和总氨基酸大大增加,表明PsAMT1 .2促进杨树根部的NH 4 +同化和代谢。与增加的总氨基酸含量一致,与盐胁迫下的WT植物相比,过表达PsAMT1.2的转基因植物根和叶中GS1.3、GS2和Fd-GOGAT表达上调。统称为PsAMT1.2编码对盐胁迫下NH 4 +摄取和代谢至关重要的高亲和力NH 4 +转运蛋白。
更新日期:2021-05-19
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