The plant microbiome has been considered one of the most researched areas of microbial biodiversity, yet very little information is available on the microbial communities prevailing in the mushroom's ectomycorrhizosphere. Ectomycorrhizal symbioses often result in the formation of a favourable niche which enables the thriving of various microbial symbionts where these symbionts endorse functions, such as quorum sensing, biofilm formation, volatile microbial compound (VOC) production, regulation of microbial gene expression, symbiosis and virulence. The identification of hidden uncultured microbial communities around the active ectomycorrhizal zone of Astraeus from dry deciduous sal forest of Jharkhand, India was carried out using MinION Oxford Nanopore sequencing of 16S rRNA amplicons genes. High richness of Operational Taxonomic Units (1,905 OTUs) was observed. We recorded 25 distinct phyla. Proteobacteria (36%) was the most abundant phylum, followed by Firmicutes (28%), Actinobacteria (10%) and Bacteroidetes (6%), whereas Gammaproteobacteria was the most abundant class of bacterial communities in the active ectomycorrhizal zone. The ectomycorrhizosphere soil has abundant phosphate-solubilising bacteria (PSB). This is the first report of the ectomycorrhizosphere microbiome associated with Astraeus.

中文翻译：

---

编录肖拉州干燥落叶林中阿斯特拉夫活跃外生菌根区的细菌多样性

植物微生物组被认为是微生物多样性研究最广泛的领域之一，但是关于蘑菇的菌根根际普遍存在的微生物群落的信息很少。菌根菌共生通常会形成有利的生态位，从而使各种微生物共生体得以兴旺发​​展，这些共生体具有一定的功能，例如群体感应，生物膜形成，挥发性微生物化合物（VOC）产生，微生物基因表达的调控，共生和毒力。 。使用16S rRNA扩增子基因的MinION Oxford纳米孔测序技术，对印度贾坎德邦落叶落叶林中未生长的阿魏氏菌外生菌根带附近隐蔽的未培养微生物群落进行了鉴定。业务分类单位的丰富性（1，观察到905个OTU）。我们记录了25个不同的门。变形杆菌（36％）是最丰富的门，其次是Firmicutes（28％），放线菌（10％）和拟杆菌（6％），而伽马变形杆菌是活跃的外生菌根区中最丰富的细菌群落。外生菌根土壤具有丰富的磷酸盐增溶细菌（PSB）。这是与阿斯泰尔乌斯菌相关的外生菌根微生物群的首次报道。