Sparassis crispa polysaccharides (SCPs) have multiple pharmacological activities. Fermentation characteristics of SCPs and its effects on the intestinal microbes in mice remain inconclusive. In this study, SCPs were fermented by the human feces and used to administer the Kunming mice to explore the fermentation characteristics of SCPs in the intestinal tract and the effects on the intestinal microbes in mice. Results from in vitro experiments revealed that SCPs were utilized by intestinal microbiota to produce short-chain fatty acids (SCFAs). The specific monosaccharide composition of SCPs determines which SCFAs are produced. Furthermore, the colon index and villi length of the SCPs-treated mice were significantly higher compared with the control group. In addition, SCPs exhibited beneficial effect on the relative abundance and diversity of dominant bacteria in the intestinal tract, such as increasing Bacteroidetes/Firmicutes ratio and up-regulating SCFA-producing bacteria, including Bacteroidales_S24-7_group, Alloprevotella, Alistipes, Bacteroides, Butyricimonas, Parabacteroides, Lachnospiraceae_NK4A136_group and Oscillibacter. SCPs increased the abundance of genes in carbohydrate, amino acid, and energy metabolism. Our results indicate SCPs can improve the physiological indices of the colon in mice, which is likely to be associated with the increase in the relative abundance and diversity of SCFA-producing bacteria and SCFAs level produced by intestinal microbiota.

中文翻译：

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ras Spa多糖的发酵特性及其对小鼠肠道微生物的影响。

散叶松香多糖（SCPs）具有多种药理活性。SCPs的发酵特性及其对小鼠肠道微生物的影响尚无定论。在这项研究中，SCP通过人类粪便发酵，并用于管理昆明小鼠以探索SCP在肠道中的发酵特性以及对小鼠肠道微生物的影响。体外实验结果表明，肠道菌群利用SCP产生短链脂肪酸（SCFA）。SCP的特定单糖成分决定了产生哪种SCFA。此外，与对照组相比，经SCPs处理的小鼠的结肠指数和绒毛长度明显更高。此外，SCP对肠道中优势细菌的相对丰度和多样性表现出有益的影响，例如增加拟杆菌/拟杆菌比和上调产生SCFA的细菌，包括拟杆菌S24-7_group，拟变体，阿利司提，拟杆菌，Butyricimonas，副细菌类，Lachno4和Oscillibacter。SCP增加了碳水化合物，氨基酸和能量代谢中的基因丰度。我们的结果表明，SCP可以改善小鼠结肠的生理指标，这可能与SCFA产生细菌的相对丰度和多样性以及肠道菌群产生的SCFAs水平的增加有关。包括拟杆菌科S24-7_group，异养菌，阿利培斯，拟杆菌，Butyricimonas，副细菌，Lachnospiraceae_NK4A136_group和Oscillibacter。SCP增加了碳水化合物，氨基酸和能量代谢中的基因丰度。我们的结果表明，SCP可以改善小鼠结肠的生理指标，这可能与SCFA产生细菌的相对丰度和多样性以及肠道菌群产生的SCFAs水平的增加有关。包括拟杆菌科S24-7_group，异养菌，阿利培斯，拟杆菌，Butyricimonas，副细菌，Lachnospiraceae_NK4A136_group和Oscillibacter。SCP增加了碳水化合物，氨基酸和能量代谢中的基因丰度。我们的结果表明，SCP可以改善小鼠结肠的生理指标，这可能与SCFA产生细菌的相对丰度和多样性以及肠道菌群产生的SCFAs水平的增加有关。