Journal of Cardiovascular Translational Research ( IF 3.4 ) Pub Date : 2021-05-17 , DOI: 10.1007/s12265-021-10128-6 Ying Xiao 1 , Xin Song 1 , Tao Wang 1 , Xia Meng 1 , Qipu Feng 1 , Kui Li 1 , Y James Kang 1, 2
The present study was undertaken to investigate whether Cu protects vasculatures from ischemic injury in the heart. C57/B6 mice were introduced to myocardial ischemia (MI) by permanent ligation of the left anterior descending (LAD) coronary artery. Two hours post-LAD ligation, mice were intravenously injected with a Cu-albumin (Cu-alb) solution, or saline as control. At 1, 4, or 7 days post-MI, hearts were collected for further analysis. A dramatic decrease in CD31-positive endothelial cells concomitantly with abundant apoptosis, along with obstruction of blood flow, was observed in ischemic myocardium 1 day post-MI. The early Cu-alb treatment protected CD31-positive cells from apoptosis, along with a preservation of micro-vessels and a decrease in infarct size. This early vasculature preservation ensured myocardial blood perfusion and protected cardiac contractile function until 28 days post-MI. This strategy of Cu-alb treatment immediately following MI would help develop a therapeutic approach for acute heart attack patients in a clinical setting.
Graphical abstract
中文翻译:
铜通过保护缺血心肌中的内皮细胞免于凋亡来保持血管结构和功能
本研究旨在调查 Cu 是否保护脉管系统免受心脏缺血性损伤。通过永久结扎左前降支 (LAD) 冠状动脉将 C57/B6 小鼠引入心肌缺血 (MI)。LAD 结扎后两小时,给小鼠静脉注射铜白蛋白 (Cu-alb) 溶液,或生理盐水作为对照。在 MI 后 1、4 或 7 天,收集心脏用于进一步分析。在 MI 后 1 天,在缺血性心肌中观察到 CD31 阳性内皮细胞显着减少并伴有大量细胞凋亡,以及血流阻塞。早期的 Cu-alb 治疗可保护 CD31 阳性细胞免于凋亡,同时保留微血管并减少梗塞面积。这种早期的脉管系统保存确保了心肌血液灌注并保护了心脏收缩功能,直到 MI 后 28 天。这种在 MI 后立即进行 Cu-alb 治疗的策略将有助于在临床环境中为急性心脏病发作患者开发一种治疗方法。