Maclurin [(3,4-dihydroxyphenyl)-(2,4,6-trihydroxyphenyl) methanone] is a natural compound that can be extracted from white mulberry(Morus alba) and purple mangosteen(Garcinia mangostana). Maclurin is known for its dual-sided effect on reactive oxygen species (ROS). Osteosarcoma is a primary malignant tumor of the bone and is one of the most aggressive cancers. It is common especially in children and young adults and can progress into highly metastatic cancer. In this study, we investigated the anti-cancer effects of maclurin on U2OS human osteosarcoma cells. The results indicated that maclurin exerts prooxidative effects and induces apoptosis via capase-3-independent PARP regulation in U2OS human osteosarcoma cells. Maclurin also inhibits the migration of U2OS human osteosarcoma cells. Maclurin modulates two of the three major mitogen-activated protein kinases that are closely linked with cancer metastasis; that is, it activates p38 and inactivates Extracellular signal-regulated kinase. The apoptosis-inducing effects of maclurin on U2OS osteosarcoma cells were diminished by additional treatment with antioxidant N-acetyl cysteine (NAC), but the migration-inhibiting effect was not affected by NAC treatment. This further suggested the only apoptosis-inducing effect of maclurin may be strongly related to its prooxidative activity. Taken together, these results suggested that maclurin may be a strong candidate molecule as an anti-osteosarcoma agent.

中文翻译：

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Maclurin 通过促氧化活性和调节 PARP、p38 和 ERK 信号在人骨肉瘤细胞中发挥抗癌作用

Maclurin [(3,4-二羟基苯基)-(2,4,6-三羟基苯基)甲酮]是一种天然化合物，可以从白桑(Morus alba)和紫罗兰(Garcinia mangostana)中提取. Maclurin 以其对活性氧 (ROS) 的双重作用而闻名。骨肉瘤是骨的原发性恶性肿瘤，是最具侵袭性的癌症之一。它在儿童和年轻人中尤其常见，并可能发展为高度转移性癌症。在这项研究中，我们研究了 maclurin 对 U2OS 人骨肉瘤细胞的抗癌作用。结果表明，maclurin 在 U2OS 人骨肉瘤细胞中通过不依赖 capase-3 的 PARP 调节发挥促氧化作用并诱导细胞凋亡。Maclurin 还抑制 U2OS 人骨肉瘤细胞的迁移。Maclurin 调节与癌症转移密切相关的三种主要有丝分裂原活化蛋白激酶中的两种；也就是说，它激活 p38 并使细胞外信号调节激酶失活。N-乙酰半胱氨酸 (NAC)，但迁移抑制作用不受 NAC 处理的影响。这进一步表明 maclurin 唯一的细胞凋亡诱导作用可能与其促氧化活性密切相关。总之，这些结果表明 maclurin 可能是作为抗骨肉瘤药物的强有力的候选分子。