Abstract—

Among actinobacteria associated with ants Camponotus vagus, Formica cunicularia, and Lasius niger, their nests, and intact soils, cellulolytic activity was screened in medium with carboxymethyl cellulose (CMC) using Congo Red dye. Assessment of culture fluids of tested strains on the CMC-plates and comparing them with solutions of the commercial cellulase preparation allowed for quantifying their activity. Data were confirmed by evaluating endo-1,4-glucanase activity using the 3,5-dinitrosalicylic acid (DNS) method. The three most active strains of actinobacteria were isolated from ants Camponotus vagus, Lasius niger, and Formica cunicularia and belonged to the genus Streptomyces. The 16S rDNA sequences of the strains Streptomyces sp. Fu₂, Streptomyces sp. Ln₉, and Streptomyces sp. Pe₃ were deposited in the GenBank under accession numbers MN227506, MN227508, and MG 705182, respectively. Specific endoglucanase and β-glucosidase activity of these strains corresponded to that of cellulose- and lignin-destroying streptomycetes described in the literature.

中文翻译：

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与蚂蚁相关的放线菌对纤维素的破坏活性研究

摘要-

在与蚂蚁Camponotus vagus，Formica cunicularia和Lasius niger，它们的巢和完整土壤相关的放线菌中，使用刚果红染料在羧甲基纤维素（CMC）培养基中筛选纤维素分解活性。评估CMC平板上测试菌株的培养液，并将其与商业纤维素酶制剂的溶液进行比较，以定量其活性。通过使用3,5-二硝基水杨酸（DNS）方法评估内切1,4-葡聚糖酶活性来确认数据。三种放线菌活性最强的菌株是从迷走蚁（Camponotus vagus），黑松（Lasius niger）和胶蚁（Formica cunicularia）中分离的，属于该属。链霉菌。链霉菌（Streptomyces sp）的16S rDNA序列。富₂，链霉菌 属。Ln ₉和链霉菌 sp。Pe ₃分别以登录号MN227506，MN227508和MG 705182保藏在GenBank中。这些菌株的特异性内切葡聚糖酶和β-葡萄糖苷酶活性与文献中描述的破坏纤维素和木质素的链霉菌的活性相对应。