Sustainable zinc (Zn) management strategies are needed to improve cereal yield production, particularly under tropical savannah conditions. Inoculation with plant growth-promoting bacteria, such as Azospirillum brasilense, could be a useful and sustainable strategy to enhance agronomic Zn use efficiency (AZnUE), leading to better cereal development. This study was developed to explore the effect of seed inoculation with A. brasilense combined with Zn application rates on maize-wheat crop development, AZnUE and grain yield. The field experiments were conducted during four cropping seasons, using five Zn application rates (0–8 kg Zn ha⁻¹), with maize grown in the first season, and two seed inoculation treatments (without or with A. brasilense) applied to both maize and wheat crops. Our results showed that inoculation was effective in promoting maize and wheat development with higher AZnUE (an increase of 76% and 17% for maize and wheat, respectively), leading to a greater yield (an increase of 6% for the maize and wheat harvests). The estimated applied Zn rates (between 4 and 5 kg Zn ha⁻¹) resulted in higher maize-wheat grain yields. Our study explored new perspectives of A. brasilense inoculation related to nutrient uptake and use efficiency under different seasonal climatic conditions. First, we examined the positive effect of A. brasilense on maize and wheat Zn uptake and AZnUE in tropical Zn-deficient soils. In addition, we examined the positive effect of A. brasilense inoculation on maize and wheat development and grain yield under harsh climatic conditions such as in the Brazilian savannah.

需要可持续的锌（Zn）管理策略来提高谷物的产量，特别是在热带稀树草原条件下。用促进植物生长的细菌（例如巴西细螺旋藻）进行接种可能是提高农艺锌利用效率（AZnUE）的有益且可持续的策略，从而促进谷物的更好发育。开展这项研究的目的是为了探索接种巴西青霉种子并结合锌的施用量对玉米-小麦作物生长，AZnUE和谷物产量的影响。田间试验是在四个作物季节中进行的，使用五个锌的施用量（0-8 kg Zn ha ^-1），玉米在第一个季节生长，并且两次接种接种处理（不使用或不使用A. brasilense））同时应用于玉米和小麦作物。我们的结果表明，接种能有效促进玉米和小麦的发育，其AZnUE较高（玉米和小麦分别增加76％和17％），从而导致更高的产量（玉米和小麦收成增加6％） ）。估计的锌施用量（4至5 kg Zn ha ^-1之间）导致较高的玉米小麦籽粒产量。我们的研究探索了在不同季节气候条件下与土壤养分吸收和利用效率相关的巴西盲肠接种的新观点。首先，我们研究了巴西农杆菌对热带缺锌土壤中玉米和小麦锌吸收和AZnUE的积极作用。此外，我们还研究了巴西拟南芥的积极作用 在恶劣的气候条件下（例如在巴西大草原上）接种玉米和小麦，以及谷物产量。