The number and classification of tribes in the leafhopper subfamily Typhlocybinae are not yet fully clear, and molecular data has recently been used to help resolve the problem. In this study, the mitochondrial genomes of Mitjaevia shibingensis Chen, Song & Webb, 2020 and M. dworakowskae Chen, Song & Webb, 2020 of the tribe Erythroneurini (Cicadellidae, Typhlocybinae) were sequenced. Most protein-coding genes (PCGs) start with ATN and end with TAA or TAG, and the AT content of these three codons were found differ from previous results that show that the first codon has the highest incidence. Two rRNA genes are highly conserved, and the AT content in 16S is higher than that of 12S. The nucleotide diversity and genetic distance among 13 PCGs of the four tribes from Typhlocybinae show that Empoascini nucleotide diversity is significantly less than in the other three tribes, and have the largest distance from the others, while Typhlocybini and Zyginellini have the smallest distance, indicating that the relationship between the two is the closest. The nad2, nad4, nad4L, and nad5 genes have greater nucleotide diversity, showing potential for use as the main markers for species identification. The phylogenetic analysis yielded a well-supported topology with most branches receiving maximum support and a few branches pertaining to relationships within Zyginellini and Typhlocybini receiving lower support. The species of these two tribes are intertwined, and it was impossible to resolve them into separate branches. In addition, the tribes Empoascini and Erythroneurini were recovered as monophyletic, and Alebrini was placed at the base of the tree as the most primitive. These results are broadly in line with other molecular phylogenetical studies which differ from traditional morphological classification.

中文翻译：

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评估了两个拟南芥叶蝉（半翅目，Ci科，犬瘟热，Erythroneurini）的线粒体全基因组，并对其系统发育状况和部落之间的关系进行了评估

叶蝉亚科Typhlocybinae中部落的数量和分类尚不完全清楚，最近已使用分子数据来帮助解决该问题。在这项研究中，对线虫基因组的线粒体基因组进行了测序，其中包括棉铃虫（Micjaevia shibingensis）Chen，Song＆Webb，2020和M. dworakowskae Chen，Song＆Webb，2020的Erythroneurini（Cicadellidae，Typhlocybinae）部落。大多数蛋白质编码基因（PCG）以ATN开头，以TAA或TAG结尾，发现这三个密码子的AT含量与以前的结果不同，后者表明第一个密码子的发生率最高。两个rRNA基因高度保守，并且16S中的AT含量高于12S。香蒲属四个部落的13个PCG之间的核苷酸多样性和遗传距离表明，Empoascini核苷酸多样性显着小于其他三个部落，且彼此的距离最大，而香蒲和齐氏菌的距离最小，表明两者之间的关系是最接近的。nad2，nad4，nad4L和nad5基因具有更大的核苷酸多样性，显示出有潜力用作物种鉴定的主要标记。系统发育分析得出了一个得到良好支持的拓扑结构，其中大多数分支获得了最大的支持，而少数与Zyginellini和Typhlocybini中的关系有关的分支获得了较低的支持。这两个部落的物种交织在一起，不可能将它们分解为单独的分支。此外，部落Empoascini和Erythroneurini被恢复为单一物种，而Alebrini被认为是最原始的树根。这些结果与其他分子系统发育研究大体上一致，这些研究不同于传统的形态学分类。