Abstract

The study aimed to investigate the potency of Leptospermum petersonii, Eucalyptus citriodora, Pelargonium graveolens, and Pelargonium roseum essential oils (EOs) against Streptococcus sanguinis and Streptococcus salivarius dental isolates. To examine the variation in sensitivity among strains of different origin, isolates from symptomatic and asymptomatic root canal infections, as well as the reference, strains S. sanguinis ATCC 10556 and S. salivarius ATCC 9222 were used in the study. EOs were chemically characterised (GC/MS). Their antibacterial and antibiofilm properties were detected by microdilution and CV assay respectively. Statistical analysis was performed by One way ANOVA. Examined bacterial strains, regardless clinical or ATCC, exhibited the highest sensitivity to L. petersonii and E. citriodora EOs (MIC ranged 0.53±0.00 - 5.88±1.27 mg/ml and 0.08±0.03 - 9.37±1.25 mg/ml respectively), while two other EOs were less efficient. Comparison of MIC values showed that sensitivity of the isolates recovered from symptomatic infections was the least, while the susceptibility of asymptomatic isolates and especially of reference strains were higher. EO inhibition of biofilm formation differed between clinical isolates of S. salivarius and their reference strain. Inhibition was higher in the case of S. salivarius ATCC 9222 (84 %-89 %) in comparison to that of clinical isolates (24 %-75 %). Regardless of the strains tested, E. citriodora exhibited the highest antibiofilm potential. To conclude: L. petersonii induced notable antibacterial potential, while E. citriodora EO proved promising antibacterial and antibiofilm activity. Due to the variable sensitivity of the isolates to EOs, it is suggested to screen activity on a large number of strains, especially from symptomatic infections.

摘要

这项研究旨在调查petersonii，桉树，天竺葵，天竺葵精油（EOs）对血链球菌和唾液链球菌分离株的效力。为了检查不同来源菌株之间的敏感性差异，从有症状和无症状根管感染分离株以及参考菌株中，使用了桑吉氏葡萄球菌ATCC 10556和唾液链球菌ATCC 9222菌株。对EO进行了化学表征（GC / MS）。分别通过微量稀释和CV分析检测了它们的抗菌和抗生物膜特性。通过单向方差分析进行统计分析。不论临床或ATCC，经检查的细菌菌株均表现出对L. petersonii和E.citriodora EOs的最高敏感性（MIC范围为0.53±0.00-5.88±1。分别为27 mg / ml和0.08±0.03-9.37±1.25 mg / ml），而其他两种EO效率较低。MIC值的比较表明，从有症状感染中分离出的分离株的敏感性最低，而无症状分离株（尤其是参考菌株）的敏感性更高。唾液链球菌的临床分离株与其参考菌株之间对生物膜形成的EO抑制作用有所不同。与临床分离株（24％-75％）相比，唾液链球菌ATCC 9222（84％-89％）的抑制作用更高。不管测试的菌株如何，柠檬曲霉都表现出最高的抗生物膜潜力。结论：L. petersonii诱导了显着的抗菌潜能，而citriodora EO被证明具有良好的抗菌和抗生物膜活性。由于分离株对EO的敏感性不同，