Metastasis is inevitable in about 30% of patients with primary renal cell carcinoma after nephrectomy treatment. APOBEC1 complementation factor (A1CF), an RNA binding protein, participates in tumor progressions such as growth, apoptosis, differentiation, and invasion. Here, we explored biological functions of A1CF and provided a new insight into renal cell carcinoma metastasis. Wound healing assay was conducted to detect migration in A1CF overexpression and knockdown stable cell lines. Quantitative PCR and western blot assays were utilized to test transcriptional and translation levels of A1CF and SMAD3 in A1CF overexpression and knockdown renal carcinoma cells. Nuclear and cytoplasmic protein separation assays were conducted to evaluate the subcellular distribution of A1CF and SMAD3. Immunoprecipitation assay was conducted to detect the interaction between A1CF and SMAD3. Our study demonstrated A1CF overexpression facilitated cell migration in renal carcinoma cells. A1CF deficiency downregulated expression of SMAD3, Snail1, and N-cadherin. In addition, A1CF promoted nucleus translocation of SMAD3 and interacted with SMAD3. SMAD3 knockdown attenuated cell migration induced by A1CF overexpression. Our study suggested A1CF facilitated cell migration by promoting nucleus translocation of SMAD3 in renal cell carcinoma cells.

大约 30% 的原发性肾细胞癌患者在接受肾切除术治疗后转移是不可避免的。APOBEC1 互补因子 (A1CF) 是一种 RNA 结合蛋白，参与肿瘤进展，如生长、凋亡、分化和侵袭。在这里，我们探索了 A1CF 的生物学功能，并提供了对肾细胞癌转移的新见解。进行伤口愈合试验以检测 A1CF 过表达和敲低稳定细胞系中的迁移。定量 PCR 和蛋白质印迹分析用于测试A1CF和SMAD3 的转录和翻译水平在 A1CF 过表达和敲低肾癌细胞中。进行核和细胞质蛋白分离测定以评估 A1CF 和 SMAD3 的亚细胞分布。进行免疫沉淀测定以检测A1CF和SMAD3之间的相互作用。我们的研究表明 A1CF 过表达促进了肾癌细胞中的细胞迁移。A1CF 缺乏下调 SMAD3、Snail1 和 N-钙粘蛋白的表达。此外，A1CF 促进 SMAD3 的细胞核易位并与 SMAD3 相互作用。SMAD3 敲低减弱了 A1CF 过表达诱导的细胞迁移。我们的研究表明，A1CF 通过促进肾细胞癌细胞中 SMAD3 的细胞核易位来促进细胞迁移。