Current diagnostic techniques for the detection of the small hive beetle (SHB), Aethina tumida are limitedly available and not cost effective. More sensitive pragmatic methods are preferred for early detection. To improve diagnostics, we focused on sampling techniques for SHB frass, as an indicator for SHB presence in a honey bee colony. In this study, we successfully tested a novel approach of employing swab sample collection of frass for real-time PCR detection of SHB.

中文翻译：

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小蜂巢甲虫的检测：作为 DNA 来源的废物

用于检测小蜂巢甲虫（SHB）的目前的诊断技术，Aethina tumida是有限可用的和不符合成本效益。更敏感的实用方法更适合早期检测。为了改进诊断，我们专注于 SHB frass 的采样技术，作为蜂群中 SHB 存在的指标。在这项研究中，我们成功地测试了一种采用 frass 拭子样本收集进行 SHB 实时 PCR 检测的新方法。