Salusin-β (Sal-β), which originates from preprosalusin, is a multifunctional hormone with a peptide structure. Sal-β exists in the hypothalamus and can stimulate the pituitary gland. The present study was conducted to determine the effects of Sal-β on hormones that play roles in the male reproductive system. Forty male Wistar Albino rats were used in the study. No infusions were performed on the control group, and infusions were applied to the infusion groups (artificial cerebrospinal fluid to the sham group, 2 and 20 nM Sal-β to the experimental group) through intracerebroventricular infusion for 7 days at 10 μl/hour rate. The animals were decapitated after 7 days of infusion; and the hypothalamus, testicles, and blood tissue samples were collected. The gonadotropin-releasing hormone (GnRH) mRNA levels were determined from the hypothalamus tissues by using the Real Time-PCR Method, and the serum luteinizing hormone (LH), follicle-stimulating hormone (FSH) and testosterone levels were determined using the ELISA method. Also, Hematoxylin-Eosin Staining Method was used for histopathological evaluations in the testicle tissues. As a result, Sal-β infusion increased GnRH mRNA levels in hypothalamus tissues (p < 0.05) besides, serum LH, FSH, and testosterone levels of the rats were higher at significant levels following Sal-β infusion compared to the control and sham group (p < 0.05). In the histological examination of the testicle tissues, Sal-β application was found to decrease the seminiferous tubule diameter and germinal epithelial thickness (p < 0.05). This evidence is the first, indicating that Sal-β, which is administered to male rats with central infusion, stimulates hypothalamus and pituitary tissues, and causes increased secretion of male reproductive hormones.

Salusin-β (Sal-β) 起源于 preprosalusin，是一种具有肽结构的多功能激素。Sal-β存在于下丘脑中，可刺激脑垂体。本研究旨在确定 Sal-β 对在男性生殖系统中发挥作用的激素的影响。四十男Wistar 白化病研究中使用了大鼠。对照组不进行输液，输液组（假手术组为人工脑脊液，实验组为2和20 nM Sal-β）以10 μl/小时的速度脑室内输液7天。 . 输注7天后将动物断头；并采集下丘脑、睾丸和血液组织样本。采用Real Time-PCR法测定下丘脑组织促性腺激素释放激素（GnRH）mRNA水平，采用ELISA法测定血清促黄体激素（LH）、促卵泡激素（FSH）和睾酮水平. 此外，苏木精-伊红染色法用于睾丸组织的组织病理学评估。因此，Sal-β输注增加了下丘脑组织中的GnRH mRNA水平（p < 0.05），并且与对照组和假手术组相比，Sal-β输注后大鼠的血清LH、FSH和睾酮水平显着升高（p < 0.05） ）。在睾丸组织的组织学检查中，发现 Sal-β 应用降低了曲细精管直径和生发上皮厚度 (p < 0.05)。这一证据是第一个，表明通过中枢输注给予雄性大鼠的 Sal-β 刺激下丘脑和垂体组织，并导致雄性生殖激素分泌增加。05)。在睾丸组织的组织学检查中，发现 Sal-β 应用降低了曲细精管直径和生发上皮厚度 (p < 0.05)。这是第一个证据，表明通过中枢输注给予雄性大鼠的 Sal-β 刺激下丘脑和垂体组织，并导致雄性生殖激素分泌增加。05)。在睾丸组织的组织学检查中，发现 Sal-β 应用降低了曲细精管直径和生发上皮厚度 (p < 0.05)。这一证据是第一个，表明通过中枢输注给予雄性大鼠的 Sal-β 刺激下丘脑和垂体组织，并导致雄性生殖激素分泌增加。