The aim of the study was to evaluate the accuracy of microcomputed tomography (mCT) to detect dentinal cracks when compared with scanning electron microscopy (SEM) and operating microscopy (OM). Different conditions of pixel size (10 or 17 μm), sample moisture (dry/moist), and transillumination (with/without) were evaluated. Additionally, the influence of the dentinal defect width on its detection was analyzed. The root canals of human mandibular incisors were prepared with the Reciproc R40 instrument (VDW, Munich, Germany). The roots were sectioned 5 and 10 mm from the apex, and mCT scans of middle and apical segments were performed at two pixel sizes: 10 μm and 17 μm, under dry and moist conditions (groups: 10dry, 10moist, 17dry, and 17moist). The operating microscope was used with and without transillumination (groups: OMTrans and OM). Findings showed that accuracy was moderate for the 10dry, 10moist, and OMTrans groups, poor for OM and very poor for 17dry and 17moist. The thickness of the dentin crack significantly influenced its detection by mCT using the resolution of 10 μm in both dry and wet conditions (), 17 μm in the dry condition (), and by the operating microscope using transillumination (). Some cracks visualized in SEM were not detected by mCT and an operating microscope. Not only the mCT resolution but also the sample moisture condition and the dentinal crack width can significantly influence its detection.

中文翻译：

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微计算机断层扫描检测牙本质裂纹的准确性：扫描电子和手术显微镜的相关研究

该研究的目的是评估显微计算机断层扫描 (mCT) 与扫描电子显微镜 (SEM) 和手术显微镜 (OM) 相比检测牙本质裂纹的准确性。像素尺寸（10或17的不同条件 μ米），样品水分（干/湿），并透照（有/无）进行了评价。此外，还分析了牙本质缺损宽度对其检测的影响。用 Reciproc R40 仪器（VDW，慕尼黑，德国）制备人下颌切牙的根管。在距根尖 5 和 10 毫米处对根部进行切片，并以两种像素尺寸对中段和根尖段进行 mCT 扫描：10  μ m 和 17  μm，在干燥和潮湿条件下（组：10dry、10moist、17dry 和 17moist）。手术显微镜使用和不使用透照（组：OMTrans 和 OM）。结果表明，10dry、10moist 和 OMTrans 组的准确度适中，OM 较差，17dry 和 17moist 非常差。牙本质裂纹的厚度显着影响其在干湿条件下使用 10 μm分辨率的 mCT 检测 （),干燥条件下为17  μ m ()，并通过使用透照的手术显微镜 (）。mCT 和手术显微镜未检测到在 SEM 中观察到的一些裂纹。不仅 mCT 分辨率，而且样品水分条件和牙本质裂纹宽度都会显着影响其检测。