Abstract

This research aims to provide an alternative eco-friendly way to obtain silver species and assess their bactericidal activity. This study reports the synthesis of Ag₂O nanoparticles and Ag nanoparticles reduced with a green synthesis method, using a low-cost and commercial Aloe vera extract. The crystalline phases of Ag and Ag₂O nanoparticles were analyzed by X-ray diffraction. The oxidation states for both species were determined by X-ray photoelectron spectroscopy. The optical properties of the material were studied through optical absorption, which resulted in well-defined band centered at 545 nm. This result is attributed to the morphology and size of the silver nanoparticles. In addition, antibacterial tests were performed on AgNPs biosynthesized with A. vera with the Kirby–Bauer protocol on Gram-negative and Gram-positive bacteria Escherichia coli and Staphylococcus aureaus, respectively. Moreover, antifungal tests were performed with various species from Candida.

摘要

本研究旨在提供一种替代的环保方式来获取银物种并评估其杀菌活性。本研究报告了使用低成本和商业芦荟提取物合成 Ag ₂ O 纳米粒子和通过绿色合成方法还原的 Ag 纳米粒子。通过X射线衍射分析Ag和Ag ₂ O纳米颗粒的结晶相。两种物质的氧化态均通过 X 射线光电子能谱测定。通过光吸收研究了材料的光学特性，这导致了以 545 nm 为中心的明确定义的波段。该结果归因于银纳米颗粒的形态和尺寸。此外，对生物合成的 AgNPs 进行了抗菌测试。A. vera与 Kirby-Bauer 方案分别针对革兰氏阴性和革兰氏阳性细菌大肠杆菌和金黄色葡萄球菌。此外，对来自念珠菌的各种物种进行了抗真菌测试。